Introduction: The hemispheric lateralization of certain faculties in the human brain has benefits for various daily functioning. The aim of present study was to develop a Persian version of hemispheric preference test and to examine its psychometric properties. Materials and Methods: Persian version of hemispheric preference test was prepared and its psychometric properties were examined in two independent studies. In the first study, 80 students (50 females, 30 males) and in the second study, 500 students (323 females, 177 males) were selected using multi-stage cluster random sampling. The reliability of the Persian version of hemispheric preference test was assessed via Cronbach’s alpha test-retest as well as item-rest correlations and its validity was assessed via confirmatory factor analysis and criterion validity. Results: Cronbach’s alpha coefficients range (0.74- 0.88) and item-rest correlations range (0.41-0.70) according to sex variable and test-retest coefficients (0.67- 0.73) suggested that the scale has a good reliability. The results of confirmatory factor analysis indicated acceptable fitness with original two factors scale. Furthermore, the model of criterion validity coefficients showed a good validity. Conclusion: In sum, the Persian version of hemispheric preference test is a reliable and valid instrument for neuropsychological, research screening, diagnostic, and therapeutic investigations.