Introduction: Human visual system is able to recognize the objects relevant information in natural images rapidly and efficiently. In the recognition process, an object can belong to different levels of abs traction (superordinate, basic, and subordinate) in a hierarchical s tructure. However, it remains unclear whether different ques tions at levels of object categorization for identical s timulus create different activation responses in the brain or not. Materials and Methods: In order to inves tigate the relation between brain function and human behavior, three behavioral experimental s tudies have been designed with help of psychophysics’ toolbox in MATLAB R2015a. During these experiments, the participants asked to record animate, face, and animal face images as target images respect to the superordinate, basic, and subordinate levels, respectively. The experiments include seven blocks of 96 trials in superordinate (four blocks), basic (two blocks), and subordinate (one block) levels. Totally each subject has done 672 trials. Results: We observed that subjects’ reaction time were task dependent for the same images in contras t to previous s tudies. That is, images in the superordinate level were observed in the early component of reaction time whereas basic and subordinate levels emerged relatively late. In all levels, only a set of 48 target images (animal face) was analyzed. These target images were randomly mixed with other ones. Conclusion: The results showed that superordinate level is well separated from the other two levels. In other words, this level needs more general information for object recognition process than other levels. These findings sugges t that categorization of objects at different levels has done by three dis tinct neuronal circuits. Moreover, these results indicate that there are some top-down signals which change the information processing path respect to the ques tions.