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Shefaye Khatam 2018, 6 - The Spring Supplement of Shefaye Khatam 1 -: 27-27 Back to browse issues page
O27: Interaction of Cancer Stem Cells and Microglia in Glioblastoma Multiforme
Sara Abdolahi , Sepideh Ghasemi , Amir Azarhomayoun , Ali Gorji *
a. Shefa Neuroscience Research Center, Khatam-Alanbia Hospital, Tehran, Iran b. Department of Neurosurgery, Westfälische Wilhelms-Universität Münster, Münster, Germany c. Department of Neuroscience, Mashhad University of Medical Sciences, Mashhad, Iran , gorjial@uni-muenster.de
Abstract:   (45 Views)
Malignant gliomas are highly invasive brain tumors with the occurrence of multiple microglia/macrophages in the tumor microenvironment. Macrophages/microglia that found in glioma microenvironment, as tumor-infiltrating immune cells, can play a harmful role in tumor progression. In addition, glioblastoma multiforme (GBM) contains multiple aberrant differentiation and tumorigenic cancer stem cells (CSCs) that contribute to tumor heterogeneity and resistance to anti-cancer therapies. The present study was aimed to understand the interaction between microglial cells and CSCs in a co-culture system. Specific markers used for the characterization of CSCs and microglia in GBM tissues obtained from patients. Then, we applied a co-culture system consisting of permeable membrane allowing secreted soluble factors to diffuse. Measuring the effects of cytokines secreted by activated and non-activated microglia on CSCs, MTS cell proliferation assay were performed. Cell viability in CSCs treated with non-activated microglia was significantly reduced compared to the group that treated with activated microglia. The activated microglia/macrophages may interfere in the process of tumor angiogenesis, metastasis niches, recurrence and support tumour invasiveness.
 
Keywords: Microglia, Cancer Stem Cells, Cell Culture
Full-Text [PDF 241 kb]   (16 Downloads)    
Type of Study: Research --- Open Access, CC-BY-NC | Subject: Basic research in Neuroscience


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Abdolahi S, Ghasemi S, Azarhomayoun A, Gorji A. O27: Interaction of Cancer Stem Cells and Microglia in Glioblastoma Multiforme. Shefaye Khatam. 2018; 6 (S1) :27-27
URL: http://shefayekhatam.ir/article-1-1556-en.html


Volume 6 - The Spring Supplement of Shefaye Khatam 1 - Back to browse issues page
مجله علوم اعصاب شفای خاتم The Neuroscience Journal of Shefaye Khatam
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