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Shefaye Khatam 2018, 6 - The Spring Supplement of Shefaye Khatam 1 -: 41-41 Back to browse issues page
P10: Isolation and Culture of Primary Microglial Cells from Glioblastoma Patients
Sepideh Ghasemi, Sara Abdolahi, Amir Azarhomayoun, Ali Gorji *
a. Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran b. Department of Neurosurgery, Westfa¨lischeWilhelms-Universita¨t Mu¨nster, Germany c. Department of Neuroscience, Mashhad University of Medical Sciences, Mashhad, Iran , gorjial@uni-muenster.de
Abstract:   (1058 Views)

Glioblastoma multiform (GBM) is the most common and malignant form of glial tumors. GBM microenvironment contains various cell types showing characteristics of activated or dimorphic macrophages/microglia. Some of these cells provide significant help for tumor growth, while others are able to inhibit tumor progression. Microglia play a major role in brain function by monitoring tissue for pathogen via phagocytic activities. Following surgical resection, human tissue samples were transfered to the research facility. Then, mechanical and chemical dissociation and enzymatic digestion were performed. Cell pellets were resuspended in media. When reaching complete confluency, mixed glial cultures were shaked to remove astrocytes. Finally immunocytochemistry was performed on remaining cells for characterization. The cells generated from GBM surgeries were likely a mix of microglia and macrophages. A small amount of astrocytes were also presentin the culture. After confirming cell phenotype, a more detailed immunocytochemical analysis was performed. Isolated microglia express Iba1 marker. Microglia obtained from GBM can be utilise for in vitro and in vivo investigation.

Keywords: GBM, Microglia, Iba1
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Type of Study: Research --- Open Access, CC-BY-NC | Subject: Basic research in Neuroscience

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Ghasemi S, Abdolahi S, Azarhomayoun A, Gorji A. P10: Isolation and Culture of Primary Microglial Cells from Glioblastoma Patients. Shefaye Khatam. 2018; 6 (S1) :41-41
URL: http://shefayekhatam.ir/article-1-1582-en.html

Volume 6 - The Spring Supplement of Shefaye Khatam 1 - Back to browse issues page
مجله علوم اعصاب شفای خاتم The Neuroscience Journal of Shefaye Khatam
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