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:: Volume 6 - The Spring Supplement of Shefaye Khatam 1 - ::
Shefaye Khatam 2018, 6 - The Spring Supplement of Shefaye Khatam 1 -: 93-93 Back to browse issues page
P62: Agmatine Protects Against Intracerebroventricular Streptozotocin-Induced Water Maze Memory Deficit, Hippocampal ApoptosisandAkt/GSK3β Signaling Disruption
Maryam Moosavi , Amir Hossein Zarifkar * , Yaghoub Farbood , Mahin Dianat , Alireza Sarkaki , Rasoul Ghasemi
a. Department of Physiology, Shiraz University of Medical Sciences, Shiraz, Iran b. Department of Physiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences (AJUMS), Ahvaz, Iran , amirhosseinzarifkar13@yahoo.com
Abstract:   (2669 Views)

Intracerebroventricular stereptozotocin (STZ) treatment has been described as a suitable model for sporadic Alzheimer’s disease (sAD). Centrally administered STZ decreases insulin and insulin receptors in the brain and interrupts PI3/Akt signaling pathway and GSK-3β. Additionally it raises Bax/Bcl-2 ratio and prompts hippocampal apoptosis. Agmatine, a polyamine derived from L-arginine decarboxylation, is recently shown to exert some neuroprotective effects. This study aimed to assess if agmatine reverses STZ-induced memory deficits and Akt/GSK-3β signaling disruption and apoptosis in the hippocampus. Adult male Sprague-Dawely rats weighing 200-250 g were used in this study. The canules were implanted bilaterally into lateral ventricle. STZ was administered on days 1 and 3 (3 mg/kg). Agmatine treatment (40 or 80 mg/kg) was started from day 4 in an every other day manner and continued till day 14. The animal’s learning and memory capability was assessed on days 15-18 using Morris water maze. After complement of the behavioral studies the hippocampi was isolated and the amounts of hippocampal cleaved caspase 3 (the landmark of apoptosis), Bax/Bcl-2 ratio, total and phosphorylated forms of GSK-3β and p-Akt were analyzed by western blot. The results showed that agmatine in 80 but not 40 mg/kg reversed the memory loss induced by STZ. Western blot analysis revealed that STZ induced elevation of caspase-3; Bax/Bcl-2 ratio and disrupted Akt/GSK-3β signaling in the hippocampus. Agmatine prevented apoptosis and Akt/GSK-3β signaling alteration induced by STZ. This study disclosed that agmatine treatment avert not only STZ-induced memory deterioration but also hippocampal apoptosis and Akt/GSK-3β signaling interruption.

Keywords: STZ, Agmatine, Learning and Memory, Apoptosis, Akt, GSK-3β
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Type of Study: Research --- Open Access, CC-BY-NC | Subject: Basic research in Neuroscience


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Moosavi M, Zarifkar A H, Farbood Y, Dianat M, Sarkaki A, Ghasemi R. P62: Agmatine Protects Against Intracerebroventricular Streptozotocin-Induced Water Maze Memory Deficit, Hippocampal ApoptosisandAkt/GSK3β Signaling Disruption. Shefaye Khatam 2018; 6 (S1) :93-93
URL: http://shefayekhatam.ir/article-1-1675-en.html


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Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 6 - The Spring Supplement of Shefaye Khatam 1 - Back to browse issues page
مجله علوم اعصاب شفای خاتم The Neuroscience Journal of Shefaye Khatam
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