Introduction: Caloric restriction (CR) has been considered as a kind of diet therapy for some neurological diseases. This study was aimed to investigate the effect of CR on the seizure behavior induced in rats. Materials & Methods: CR was applied with deprivation of food and water for 24 hours every other day for a period of four weeks. Seizure induced by pentylenetetrazol (PTZ) injection. Animals in CR1 group were accessed caloric restriction before PTZ injection. Animals in CR2 group were accessed caloric restriction after PTZ injection. Animals in CR3 group were accessed caloric restriction before and after PTZ injection. Results: The scores of seizure behaviors were significantly decreased in groups of animals that received CR before PTZ injection. Conclusion: These findings suggest the CR reduces scores of seizure behaviour and may be helpful in the refractory epilepsy.