Long-Term Potentiation Enhanced in Juvenile Rat by Repetitive Cortical Spreading Depression
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Milad Ahmadi , Mehrnaz Banazadeh Dardashti , Mostafa Modarres Mousavi , Fariba Karimzadeh * |
a. Shefa Neuroscience Research Center, Khatam-al-Anbia Hospital, Tehran, Iran.b. School of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran. , Fariba_karimzade@yahoo.com |
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Abstract: (12846 Views) |
Introduction: Spreading depression (SD) is a bioelectrical event in the central nervous system and involves in the pathophysiology of some neurological disorders. In this present study, we indicate enhancement of long-term potentiation of hippocampus tissue in juvenile rats faced to cortical spreading depression repetitively. Materials and Methods: Silver recording electrodes as well as a cannula were implanted over the brain of juvenile rats. Repetitive cortical SD events were induced by KCl 3 M weekly injection through the cannula. The brains were removed after 4 weeks. Transverse sections were prepared and incubated in artificial cerebrospinal fluid. Single electrical stimulations were applied through a bipolar electrode placed on to the hippocampal Schaffer collaterals. The field excitatory postsynaptic potentials (fEPSP) were elicited by adjusting the intensity of stimulation to 50% of that at which population spikes began to appear. Results: Repetitive SD enhanced the long-term potentiation in CA1 hippocampal area. The data indicate that repetitive cortical SD in juvenile rats significantly increases the amplitude of the fEPSP form the baseline. Conclusion: This may clarify the pathophysiology of memory deficit were seen in some neurological disorders in children. |
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Keywords: Long-Term Potentiation, Learning, Memory, Neuronal Plasticity, Hippocampus |
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Full-Text [PDF 631 kb]
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Type of Study: Research --- Open Access, CC-BY-NC |
Subject:
Neurophysiopathology
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