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:: Volume 2, Issue 2 (Spring - 2014) ::
Shefaye Khatam 2014, 2(2): 47-55 Back to browse issues page
Neurogenic Differentiation of Rat Bone Marrow Stromal Cells by the Non Toxic Factors of Bioactive Substance as an Inducer
Parastoo Barati , Marzieh Darvishi , Taghi Tiraihi *, Taher Doroudi
Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran. , takialtr@modares.ac.ir
Abstract:   (4213 Views)

Introduction: Despite major progress in pharmacological and surgical approaches, spinal cord injury remains a complex medical challenge. Cell replacement therapy is one of the new approaches for spinal cord injury treatment. In this study, bioactive substance TNT (a new supplement) was utilized for induction of bone marrow stromal cells (BMSCs) into neural stem cells (NSCs). Materials and Methods: The BMSCs were extracted and cultured from the femurs and tibias of adult female wistar rats. After 3 or 4 passages, these cells were preinduced into neurospheres by TNT and neural induction supplement. Then neurospheres were induced into NSCs with B27 and TNT. The NSCs were evaluated with nestin, NF68 and SOX2. Results: The outcomes indicated that BMSCs were immunoreactive to CD106 (95.7 ± 0.76), OCT4 (96.2 ± 1.3) and CD45 (0.4 ± 1.2). The optimal dose and time for application of TNT were 1 M and 6 days, respectively. Differentiated cells were able to express nestin and NF68. Conclusion: TNT as a non-toxic substance may be a good alternative for cell induction into NSCs for treatment of neurodegenerative disorders.

Keywords: Mesenchymal Stromal Cells, Cell Transplantation, Spinal Cord Injuries, Neural Stem Cells
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Type of Study: Research --- Open Access, CC-BY-NC | Subject: Basic research in Neuroscience



DOI: 10.18869/acadpub.shefa.2.2.47


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Barati P, Darvishi M, Tiraihi T, Doroudi T. Neurogenic Differentiation of Rat Bone Marrow Stromal Cells by the Non Toxic Factors of Bioactive Substance as an Inducer. Shefaye Khatam. 2014; 2 (2) :47-55
URL: http://shefayekhatam.ir/article-1-78-en.html
Volume 2, Issue 2 (Spring - 2014) Back to browse issues page
مجله علوم اعصاب شفای خاتم The Neuroscience Journal of Shefaye Khatam
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