[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit ::
:: Volume 3 - The Autumn Supplement of Shefaye Khatam 3 - ::
Shefaye Khatam 2015, 3 - The Autumn Supplement of Shefaye Khatam 3 -: 28-28 Back to browse issues page
P1: Three Dimensional Cultivation of Neural Stem Cells by Pura Matrix
Hadi Aligholi, Hassan Azari, Gholamreza Hassanzadeh, Ali Gorji *
a. Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran b. Epilepsy Research Center, Department of Neurosurgery, and Department of Neurology, Westfälische Wilhelms-Universitat Munster, Munster, Germany , gorjial@uni-muenster.de
Abstract:   (1857 Views)

There are two main methods for preparing the PuraMatrix (PM) as a three-dimensional (3-D) scaffold for supporting the cells including surface plating and encapsulation. In the surface-plating procedure, the scaffold is created by adding an ionic agent such as medium to PM. After adjusting the PH by changing the medium, cells are seeded on top of the scaffold. In the encapsulation method, PM and cells are mixed suddenly to produce a true 3-D culture.  Because PM is very acidic agent, it can be harmful for sensitive cells such as neural stem cells. We introduced a new method for solving this problem. In this novel approach, firstly, scaffold was created, then, the neural stem cells were injected into the several sites of it. In this method, the survival of the cells was significantly high than that of other methods. This method can be used in future investigations in which a 3-D culture of neural stem cells in needed.

Keywords: Neural Stem Cells, Scaffold, Tissue Engineering, Hydrogels
Full-Text [PDF 173 kb]   (467 Downloads)    
Subject: Basic research in Neuroscience


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Aligholi H, Azari H, Hassanzadeh G, Gorji A. P1: Three Dimensional Cultivation of Neural Stem Cells by Pura Matrix. Shefaye Khatam. 2015; 3 (S3) :28-28
URL: http://shefayekhatam.ir/article-1-790-en.html


Volume 3 - The Autumn Supplement of Shefaye Khatam 3 - Back to browse issues page
مجله علوم اعصاب شفای خاتم The Neuroscience Journal of Shefaye Khatam
Persian site map - English site map - Created in 0.05 seconds with 31 queries by YEKTAWEB 3991