TY - JOUR JF - The-Neuroscience-Journal-of-Shefaye-Khatam JO - Shefaye Khatam VL - 9 IS - 3 PY - 2021 Y1 - 2021/7/01 TI - Synthesis, Characterization, Evaluation of Supportive Properties, and Neuroprotective Effects of Cerium Oxide Nanoparticles as a Candidate for Neural Tissue Engineering TT - سنتز، شناسایی و ارزیابی ویژگی‌های حمایتی و اثرات محافظت عصبی نانوذرات سریم اکسید به‌عنوان کاندیدی در مهندسی بافت عصبی N2 - Introduction: Tissue engineering is a part of biotechnology that includes the development of biological implants for tissue regeneration to improve tissue or organ function. This study aimed to investigate the effect of cerium oxide nanoparticles on the interactions between adipose tissue stem cells and decellularized sciatic nerve scaffolds in rats. Materials and Methods: Rats were anesthetized by injecting a mixture of ketamine (80 mg/kg) and xylazine (10 mg/kg). Sciatic nerve fragments (15 mm) were removed above the three-pronged site in the thigh muscle and decellularized after cleaning the surrounding tissues using the Sandal method. Then, adipose tissue mesenchymal cells were implanted on the scaffold, and the growth and viability of the cells implanted on the scaffold in the presence of cerium oxide nanoparticles were measured by MTT assay. Results: The results of histological evaluations showed that the scaffolds were completely decellularized and hematoxylin/eosin and Dapi staining confirmed these results. Specialized tissue evaluation by Masson trichrome staining as well as biomechanical analysis showed that collagen and elastin fibers were relatively preserved in the extracellular matrix. Cell viability on the scaffold increased in the presence of nanoparticles. Conclusion: Cerium oxide nanoparticles increase cell stability, proliferation, and maintenance of adipose tissue mesenchymal cells and may be beneficial in the treatment of peripheral nerve lesions. SP - 55 EP - 63 AU - Arzanipur, Yasaman AU - Abdolmaleki, Arash AU - Asadi, Asadollah AU - Zahri, Saber AD - A. Department of Engineering Sciences, Faculty of Advanced Technologies, University of Mohaghegh Ardabili, Namin, Iran. b. Bio Science and Biotechnology Research center (BBRC), Sabalan University of Advanced Technologies (SUAT), Namin, Iran KW - Sciatic Nerve KW - Cerium KW - Extracellular Matrix KW - Regeneration KW - Tissue Engineering UR - http://shefayekhatam.ir/article-1-2212-en.html DO - 10.52547/shefa.9.3.55 ER -