fa P3: Neuronal Death Following Posttraumatic Excitability and Seizure تحقیقات پایه در علوم اعصاب Basic research in Neuroscience پژوهشي Research --- Open Access, CC-BY-NC <p style="text-align: center;"><span style="color: rgb(0, 0, 0); font-family: Tahoma; font-size: 12px; text-align: center; background-color: rgb(251, 250, 249);">لطفاً به چکیده انگلیسی مراجعه شود.</span></p> <p>Seizure may occur after mild traumatic brain injury (TBI), and the severity of TBI can be considered the most crucial factor for an increased risk of recurring seizures as well as for the development of posttraumatic epilepsy. However, the effect of seizures in epileptogenesis after mild TBI cannot yet be accurately confirmed. This study was designed to determine whether mild TBI increases seizure susceptibility as well as to investigate the histopathological and molecular consequences of seizure occurrence after mild TBI. Using a novel method of TBI induction, seizures were induced by sub-convulsive doses of pentylenetetrazole (PTZ) fifteen days after induction of focal mild TBI. Behavioral assessments were performed for one hour after PTZ injection and histopathological as well as molecular evaluations were performed. A significantly higher score and longer duration of seizure attacks were observed in the TBI+PTZ group compared to sham and TBI groups. A higher number of apoptotic cells was observed in the TBI+PTZ group compared to sham and TBI rats. Molecular investigations revealed higher levels of Bax/Bcl2 ratio, Caspase 3, and NF-&kappa;B in the TBI+PTZ group compared to the other animal groups. Our data indicated that seizure occurrence following mild TBI aggravates cell injury and death via activation of neuroinflammatory processes and may increase the risk of posttraumatic epilepsy.&nbsp;</p> Epileptogenesis, Hippocampus, Posttraumatic seizure, Neuronal death, Neuroinflammation 25 25 http://shefayekhatam.ir/browse.php?a_code=A-10-24-794&slc_lang=fa&sid=1 Tahereh Ghadiri Tahereh Ghadiri 100319475328460011378 100319475328460011378 No a. Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran b. School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran a. Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran b. School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran Fariba khodagholi Fariba khodagholi 100319475328460011379 100319475328460011379 No Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran Gelareh Vakilzadeh Gelareh Vakilzadeh 100319475328460011380 100319475328460011380 No a. Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran b. School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran a. Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran b. School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran Vahid Hajali Vahid Hajali 100319475328460011381 100319475328460011381 No Quchan Higher Health Education Center, Mashhad University of Medical Sciences, Mashhad, Iran Quchan Higher Health Education Center, Mashhad University of Medical Sciences, Mashhad, Iran Mohammad Sharifzadeh Mohammad Sharifzadeh 100319475328460011382 100319475328460011382 No a. Department of Toxicology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran b. School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran a. Department of Toxicology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran b. School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran Ali Gorji Ali Gorji gorjial@uni-muenster.de 100319475328460011383 100319475328460011383 Yes a. Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran b. Razavi Neuroscience Center, Razavi Hospital, Mashhad, Iran c. Department of Neurosurgery, Department of Neurology and Epilepsy Research Center, Munster University, Germany a. Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran b. Razavi Neuroscience Center, Razavi Hospital, Mashhad, Iran c. Department of Neurosurgery, Department of Neurology and Epilepsy Research Center, Munster University, Germany