en P 2: Proliferation of Neural Stem Cells Promotes in Presence of Feijoa Methanolic Extract in the Oxidative Stress Condition تحقیقات پایه در علوم اعصاب Basic research in Neuroscience پژوهشي Research --- Open Access, CC-BY-NC <table border="0" cellpadding="5px" cellspacing="2" dir="rtl" id="table_article" style="font-family: Tahoma; background-color: rgb(251, 250, 249); break-inside: avoid !important;" width="100%"> <tbody style="break-inside: avoid !important; font-size: 12px; margin-top: 0px; line-height: 2.2; margin-bottom: 0px; text-align: justify;"> <tr style="break-inside: avoid !important;"> <td class="abstractmed" colspan="2" style="break-inside: avoid !important;"> <p style="margin-top: 0px; margin-bottom: 0px; text-align: center;"><span style="color: rgb(0, 0, 0); line-height: 20.8px;">لطفاً به چکیده انگلیسی مراجعه شود.</span></p> </td> </tr> <tr style="break-inside: avoid !important;"> </tr> </tbody> </table> <p></p> <p><strong>Introduction:</strong> Now days, several studies have indicated the central nervous system has capacity for endogenous repair. But, the proliferation of endogenous neural stem cells (NSCs) is insufficient for treatment of neurodegenerative diseases. So, it sound that stimulation of endogenous NSC proliferation is essential for neuroregeneration. The <em>Acca sellowiana (Feijoa) </em>extract as herbal extract is used as antioxidant agent in antient medicine. Its fruits are rich in vitamin C, polyphenols, terpenes, tannins, steroidal saponins, flavonoids hydrocarbons, minerals, iodine and both methyl and ethyl benzoate. The aim of this study was to examine the self-proliferation and antioxidant properties of Feijoa extract on neonatal rat hippocampus-derived neural stem cells (NSCs). <strong>Materials and Methods: </strong>The NSCs were isolated and cultured. The expression of neural-specific marker, &nbsp;nestin was examined by immunocytochemistry. At first, the cells were in presence of hydrogen peroxide with 50&micro;m concentration in order to oxidative stress induction in vitro and toxicity percentage of hydrogen peroxide was examined. Then, NSCs were exposed to various concentrations (25, 50, 100 and 200 &mu;g/ml) of Acca sellowiana extract for 24 hrs. Thereafter, cell proliferation rate was assessed using MTT colorimetry assay. <strong>Results:</strong> NSCs expressed neural marker (Nestin). Proliferation rate of NSCs was increased in treated groups in comparison with control group. In addition, the results demonstrated that 100&micro;g/ml concentration was the best group for self-proliferation of NSCs. (P<0.05)<span dir="RTL">.</span> <strong>Conclusion:</strong> These finding shows that the methanolic extract of Acca sellowiana is an antioxidant compound and can promote self-proliferation and survival of NCSs in vitro<em>,</em> suggesting its potential benefits on neuroregeneration.</p> Neural stem cells, Acca sellowiana, Proliferation rate, Extract 33 33 http://shefayekhatam.ir/browse.php?a_code=A-10-24-851&slc_lang=en&sid=1 Sara Haratizadeh Sara Haratizadeh 100319475328460011783 100319475328460011783 No Student Research Committee, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran Student Research Committee, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran Javad Akhtari Javad Akhtari 100319475328460011784 100319475328460011784 No Immunogenetics Research Center, Department of Physiology and Pharmacology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran Immunogenetics Research Center, Department of Physiology and Pharmacology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran Mohammad Ali Ebrahim Zadeh Mohammad Ali Ebrahim Zadeh 100319475328460011785 100319475328460011785 No Pharmaceutical Sciences Research Center, School of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran Pharmaceutical Sciences Research Center, School of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran Alireza Abdanipour Alireza Abdanipour 100319475328460011786 100319475328460011786 No Department of Anatomy, Stem Cells Research Laboratory, Islamic Azad University, Ardabil Branch, Ardabil, Iran Department of Anatomy, Stem Cells Research Laboratory, Islamic Azad University, Ardabil Branch, Ardabil, Iran Maryam Nazm Bojnordi Maryam Nazm Bojnordi 100319475328460011787 100319475328460011787 No Department of Anatomy and Cell Biology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran Department of Anatomy and Cell Biology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran Hatef Ghasemi Hamidabadi Hatef Ghasemi Hamidabadi 100319475328460011788 100319475328460011788 Yes Immunogenetic Research Center, Mazandaran University of Medical Sciences, Sari, Iran Immunogenetic Research Center, Mazandaran University of Medical Sciences, Sari, Iran