en P 10: Safranal Attenuates Quinolinate-Induced Oxidative OLN-93 Cells Death تحقیقات پایه در علوم اعصاب Basic research in Neuroscience پژوهشي Research --- Open Access, CC-BY-NC <table border="0" cellpadding="5px" cellspacing="2" dir="rtl" id="table_article" style="font-family: Tahoma; background-color: rgb(251, 250, 249); break-inside: avoid !important;" width="100%"> <tbody style="break-inside: avoid !important; font-size: 12px; margin-top: 0px; line-height: 2.2; margin-bottom: 0px; text-align: justify;"> <tr style="break-inside: avoid !important;"> <td class="abstractmed" colspan="2" style="break-inside: avoid !important;"> <p style="font-size: 12px; margin-top: 0px; margin-bottom: 0px; text-align: center;"><span style="color: rgb(0, 0, 0); font-size: 12px; line-height: 20.8px;">لطفاً به چکیده انگلیسی مراجعه شود.</span></p> </td> </tr> <tr style="break-inside: avoid !important;"> </tr> </tbody> </table> <p></p> <p><strong>Introduction: </strong>Quinolinic acid (QA) is a product of tryptophan degradation and its pathologic accumulation has been found to induce neuroinflammatory and demyelinating diseases such as multiple sclerosis via excessive free radicals generation. Recent studies showed Safranal which is the main component of essential oil of saffron, has several pharmacological effects such as antioxidant, anti-inflammatory and neuroprotective properties. The aim of this study was evaluation of the protective effect of Safranal on oxidative OLN-93 cells death induced by QA. <strong>Materials and Methods: </strong>&nbsp;Cells were pretreated with Safranal (1-800 𝜇M) for 2 h and then were subjected to QA (8 mM) for 24 h in which the same treatments were applied. Cell viability and the parameters of redox status including the levels of intracellular reactive oxygen species (ROS), and lipid peroxidation were measured using 2-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT), 2,7-dicholorofluorecin diacetate (DCF-DA) and thiobarbituric acid assays, respectively. <strong>Results: </strong>safranal at concentration ranges of 1-800 &mu;M had no toxic effect on cell viability (p>0.05). Treatment with Safranal significantly increased cell viability following QA insult at concentrations 1-800 𝜇M (p<0.001). Cytoprotective potential of Safranal also ameliorated ROS accumulation and lipid peroxidation induced by QA. <strong>Conclusion:</strong> These data suggest that Safranal exhibits oligoprotection potential by means of alleviating oxidative stress parameters.</p> Quinolinic acid, Safranal, OLN-93 cells, Gliotoxicity 41 41 http://shefayekhatam.ir/browse.php?a_code=A-10-24-859&slc_lang=en&sid=1 Sahar Fanoudi Sahar Fanoudi 100319475328460011810 100319475328460011810 No Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Hamid R Sadeghnia Hamid R Sadeghnia hsadeghnia@gmail.com 100319475328460011811 100319475328460011811 Yes a. Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran b. Neurocognitive Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran a. Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran b. Neurocognitive Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Ameneh Veisifard Ameneh Veisifard 100319475328460011812 100319475328460011812 No Department of Biology, University of Payame Noor, Mashhad, Iran Department of Biology, University of Payame Noor, Mashhad, Iran Mohammmad Soukhatanloo Mohammmad Soukhatanloo 100319475328460011813 100319475328460011813 No Department of Biochemistry, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Department of Biochemistry, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran