en P 12: Study of the Association between Serum Level of Cystatin C and Behavioral Symptoms of 6-Hydroxydopamine – Induced Parkinsonism in Rat تحقیقات پایه در علوم اعصاب Basic research in Neuroscience پژوهشي Research --- Open Access, CC-BY-NC <table border="0" cellpadding="5px" cellspacing="2" dir="rtl" id="table_article" style="font-family: Tahoma; background-color: rgb(251, 250, 249); break-inside: avoid !important;" width="100%"> <tbody style="break-inside: avoid !important; font-size: 12px; margin-top: 0px; line-height: 2.2; margin-bottom: 0px; text-align: justify;"> <tr style="break-inside: avoid !important;"> <td class="abstractmed" colspan="2" style="break-inside: avoid !important;"> <p style="font-size: 12px; margin-top: 0px; margin-bottom: 0px; text-align: center;"><span style="color: rgb(0, 0, 0); font-size: 12px; line-height: 20.8px;">لطفاً به چکیده انگلیسی مراجعه شود.</span></p> </td> </tr> <tr style="break-inside: avoid !important;"> </tr> </tbody> </table> <p></p> <p><strong>Introduction: </strong>Parkinson&rsquo;s disease (PD) is the second most neurodegenerative disorder which is characterized by a progressive loss of dopaminergic neurons in the substantia nigra pars compacta. Clinical symptoms do not appear until approximately 70% of dopaminergic neurons and 80% of the striatal dopaminergic terminals have been lost. Thus, detecting nonclinical factors such as detecting biomarker for PD is necessary. In this study, we evaluate the serum level of Cystatin C as a possible biomarker of PD in 6-hydroxydopamine (6-OHDA)-induced Parkinsonism in rat. <strong>Materials and Methods:&nbsp; </strong>Rats were divided into two groups: Parkinson and Control. 6-OHDA was administered by stereotaxic surgery into forebrain bundle. Severity of the Parkinsonism was evaluated by Apomorphine (APO)-induced rotational test at the third and sixth week&rsquo;s post-surgery. Also, serum level of Cystatin C was measured before surgery and at the third and sixth weeks post-surgery. <strong>Results: </strong>Although rats of control group didn&rsquo;t show a significant response to APO, rats of parkinson group showed significant rotations. The rotations at the sixth week&rsquo;s post-surgery were significantly more than the rotations at the third week&rsquo;s post-surgery. However, there was no significant difference between serum level of Cystatin C in rats of control and parkinson group. Also, there was no difference between serum level of Cystatin C in rats of parkinson group before and after the surgery. There was no difference between serum level of Cystatin C and severity of symptoms in rats of parkinson group. <strong>Conclusion: </strong>Our data show that in 6-OHDA animal model of PD, serum level of Cystatin C cannot predict onset or progress of PD and therefore this compound cannot consider as a biomarker for PD.&nbsp;</p> 6-hydroxydopamine, Animal model of parkinson, Apomorphine-induced rotational test, Cystatin C 43 43 http://shefayekhatam.ir/browse.php?a_code=A-10-24-861&slc_lang=en&sid=1 Ali Sarbazi Golezari Ali Sarbazi Golezari ali@doctor.com 100319475328460011819 100319475328460011819 Yes a. Student Research Committee, Qazvin University of Medical Sciences, Qazvin, Iran b. Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran a. Student Research Committee, Qazvin University of Medical Sciences, Qazvin, Iran b. Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran Nafiseh Rastgoo Nafiseh Rastgoo 100319475328460011820 100319475328460011820 No Student Research Committee, Qazvin University of Medical Sciences, Qazvin, Iran Student Research Committee, Qazvin University of Medical Sciences, Qazvin, Iran Ayda Faraji Ayda Faraji 100319475328460011821 100319475328460011821 No Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran Tahereh Dargahi Tahereh Dargahi 100319475328460011822 100319475328460011822 No Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran Gilda Khandan Gilda Khandan 100319475328460011823 100319475328460011823 No Student Research Committee, Qazvin University of Medical Sciences, Qazvin, Iran Student Research Committee, Qazvin University of Medical Sciences, Qazvin, Iran Arvin Babayan Arvin Babayan 100319475328460011824 100319475328460011824 No Student Research Committee, Qazvin University of Medical Sciences, Qazvin, Iran Student Research Committee, Qazvin University of Medical Sciences, Qazvin, Iran Hashem Haghdoost Yazdi Hashem Haghdoost Yazdi 100319475328460011825 100319475328460011825 No Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran