en P 14: CSF NGF/IL-6 Ratio: a Useful Marker for the Evaluation of Progesterone Efficacy in Traumatic Brain Injury تحقیقات پایه در علوم اعصاب Basic research in Neuroscience پژوهشي Research --- Open Access, CC-BY-NC <table border="0" cellpadding="5px" cellspacing="2" dir="rtl" id="table_article" style="font-family: Tahoma; background-color: rgb(251, 250, 249); break-inside: avoid !important;" width="100%"> <tbody style="break-inside: avoid !important; font-size: 12px; margin-top: 0px; line-height: 2.2; margin-bottom: 0px; text-align: justify;"> <tr style="break-inside: avoid !important;"> <td class="abstractmed" colspan="2" style="break-inside: avoid !important;"> <p style="font-size: 12px; margin-top: 0px; margin-bottom: 0px; text-align: center;"><span style="color: rgb(0, 0, 0); font-size: 12px; line-height: 20.8px;">لطفاً به چکیده انگلیسی مراجعه شود.</span></p> </td> </tr> <tr style="break-inside: avoid !important;"> </tr> </tbody> </table> <p></p> <p><strong>Introduction:</strong> Following our previous studies on the neuroprotective effects of progesterone and cytokines such as IL-6 (IL-6) is involved in the inflammatory response, to examine whether changes in IL-6 and Nerve grows factor(NGF) concentrations in CSF can responsible for the neuroprotective effects of progesterone after traumatic brain injury(TBI). <strong>Materials and Methods:</strong> Female ovariectomized rats were divided into 9 groups: intact (pro estrous and non-proestrous), sham, TBI and 4 groups treated by vehicle or different doses of progesterone, including: vehicle, LP (low dose of progesterone, 1.7 mg/kg), HP (high dose of progesterone, 8 mg/kg), IVC (implant vehicle capsules, 10-20 ng/ml) and IPC (Implant low dose of progesterone capsules, 10-20 ng/ml). In groups receiving hormone or vehicle, treatment was administered as a single dose of intraperitoneally 30 minutes or implant capsules 6 hours following a diffuse TBI that was induced by Marmarou&rsquo;s method. The levels of biomarkers in CSF were measured at 48 h after the TBI. <strong>Results:</strong> Both doses of progesterone reduced CSF levels of IL-6 compared with vehicle group (p<0.05, p<0.001, respectively), but the difference between CSF levels of NGF in progesterone and vehicle was not significant. After trauma, although the ratio of NGF to IL-6 significantly higher in the progesterone groups than in the vehicle group (p<0.05). The CSF level of IL-6 was reduced in IPC group, compared with IVC group (p<0.05), but the CSF level of NGF is increased (p<0.05). The NGF/IL-6 ratio in IPC group was 4.13 higher following administration of vehicle (1.16, p<0.001) levels, and the highest maximum NGF/IL-6 ratio is in the same group. <strong>Conclusion:</strong> Based on our findings, we conclude that individual measure these two indicators to evaluate the effects of drugs may be not useful, but CSF NGF/IL-6 ratio might be a better marker for determine the effectiveness of drugs.</p> TBI, Progesterone, NGF, IL-6, CSF NGF/IL-6 ratio 45 45 http://shefayekhatam.ir/browse.php?a_code=A-10-24-863&slc_lang=en&sid=1 Sara Shiraz Pour Sara Shiraz Pour khaksar38@yahoo.co.uk 100319475328460011830 100319475328460011830 Yes Neuroscience Research Center, and Physiology Department, Kerman University of Medical Sciences, Kerman, Iran Neuroscience Research Center, and Physiology Department, Kerman University of Medical Sciences, Kerman, Iran Mohammad Khaksari Mohammad Khaksari 100319475328460011831 100319475328460011831 No Neuroscience Research Center, and Physiology Department, Kerman University of Medical Sciences, Kerman, Iran Neuroscience Research Center, and Physiology Department, Kerman University of Medical Sciences, Kerman, Iran Neda Salmani Neda Salmani 100319475328460011832 100319475328460011832 No Neuroscience Research Center, and Physiology Department, Kerman University of Medical Sciences, Kerman, Iran Neuroscience Research Center, and Physiology Department, Kerman University of Medical Sciences, Kerman, Iran Zahra Soltany Zahra Soltany 100319475328460011833 100319475328460011833 No Neuroscience Research Center, and Physiology Department, Kerman University of Medical Sciences, Kerman, Iran Neuroscience Research Center, and Physiology Department, Kerman University of Medical Sciences, Kerman, Iran Alireza Raji Alireza Raji 100319475328460011834 100319475328460011834 No Neuroscience Research Center, and Physiology Department, Kerman University of Medical Sciences, Kerman, Iran Neuroscience Research Center, and Physiology Department, Kerman University of Medical Sciences, Kerman, Iran