en P 15: Forced Exercise Attenuates Neuropathic Pain in Chronic Constriction Injury Male Rat: An Approach to Oxidative Stress and Inflammation تحقیقات پایه در علوم اعصاب Basic research in Neuroscience پژوهشي Research --- Open Access, CC-BY-NC <table border="0" cellpadding="5px" cellspacing="2" dir="rtl" id="table_article" style="font-family: Tahoma; background-color: rgb(251, 250, 249); break-inside: avoid !important;" width="100%"> <tbody style="break-inside: avoid !important; font-size: 12px; margin-top: 0px; line-height: 2.2; margin-bottom: 0px; text-align: justify;"> <tr style="break-inside: avoid !important;"> <td class="abstractmed" colspan="2" style="break-inside: avoid !important;"> <p style="font-size: 12px; margin-top: 0px; margin-bottom: 0px; text-align: center;"><span style="color: rgb(0, 0, 0); font-size: 12px; line-height: 20.8px;">لطفاً به چکیده انگلیسی مراجعه شود.</span></p> </td> </tr> <tr style="break-inside: avoid !important;"> </tr> </tbody> </table> <p></p> <p><strong>Introduction: </strong>Peripheral and central nerve injuries cause chronic neuropathic pain in many injured people besides motor disability. Exercise, as a behavioral and non-pharmacological treatment, has beneficial effects on people&rsquo;s general health both for healthy and sick people. Therefore, this study was conducted to examine the effects of exercise on neuropathic pain induced by chronic constriction injury (CCI) of the sciatic nerve. <strong>Materials and Methods: </strong>Wistar male rats weighing 200 &plusmn; 20 g were randomly divided into five groups (normal, sham, CCI, pre-CCI exercise, and post-CCI exercise group). Once the rats were anesthetized, their sciatic nerve was legated to induce CCI, and they were then housed in separate cages. The rats ran on treadmill at a moderate speed for 3 weeks. Mechanical allodynia and thermal hyperalgesia were determined using von Frey Filament and plantar test, respectively. TNF-&alpha;, malondialdehyde, and total antioxidant capacity were measured using Western blot test, thiobarbituric acid, and ferric reducing ability of plasma (FRAP) respectively. <strong>Results:</strong> The mechanical allodynia (P<0.05) and thermal hyperalgesia (P<0.01) in the CCI group were significantly higher than those in the sham group. Exercise after CCI significantly reduced (P<0.01) mechanical allodynia and thermal hyperalgesia (P<0.05) against those in the CCI group. Moreover, the level of FRAP in the CCI group was significantly (P<0.01) lower than that in the sham group, and the level of FRAP in the post-CCI group increased significantly (P<0.05) against that in the CCI group. The level of MDA did not differ significantly between groups. Level of TNF-&alpha; increased significantly in the CCI group (P<0.001) compared to sham group and decreased significantly in the post-CCI group (P<0.01) against that in the CCI group. <strong>Conclusion:</strong> Exercise reduces mechanical allodynia and thermal hyperalgesia induced by CCI. These effects probably mediated by increasing the total antioxidant capacity and reducing the TNF-&alpha; inflammation factor.&nbsp; &nbsp;&nbsp;</p> CCI, TNF-α, Treadmill exercise, Neuropathic pain, Oxidative stress 46 46 http://shefayekhatam.ir/browse.php?a_code=A-10-24-864&slc_lang=en&sid=1 Nasroallah Moradi-kor Nasroallah Moradi-kor 100319475328460011835 100319475328460011835 No a. Research Center of Physiology, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran b. Student Research Committee, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran a. Research Center of Physiology, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran b. Student Research Committee, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran Hossein Ali Safakhah Hossein Ali Safakhah 100319475328460011836 100319475328460011836 No Laboratory of Pain Research, Research Center and Department of Physiology, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran Laboratory of Pain Research, Research Center and Department of Physiology, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran Atiyeh Bazargani Atiyeh Bazargani 100319475328460011837 100319475328460011837 No Student Research Committee, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran Student Research Committee, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran Ahmad Reza Bandegi Ahmad Reza Bandegi 100319475328460011838 100319475328460011838 No Department of Biochemistry, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran Department of Biochemistry, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran Hamid Gholami Pourbadie Hamid Gholami Pourbadie 100319475328460011839 100319475328460011839 No Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, Iran Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, Iran Baharak Khoshkholgh Sima Baharak Khoshkholgh Sima 100319475328460011840 100319475328460011840 No Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, Iran Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, Iran Ali Ghanbari Ali Ghanbari aligh88@gmail.com 100319475328460011841 100319475328460011841 Yes Research Center of Physiology, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran Research Center of Physiology, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran