en P 33: Effect of Alcoholic Extract of Rosmarinus officinalis L. and Rosmarinic Acid on Inflammation Induced by Chronic Constriction Injury (CCI) Model of Neuropathic Pain in Rats تحقیقات پایه در علوم اعصاب Basic research in Neuroscience پژوهشي Research --- Open Access, CC-BY-NC <table border="0" cellpadding="5px" cellspacing="2" dir="rtl" id="table_article" style="font-family: Tahoma; background-color: rgb(251, 250, 249); break-inside: avoid !important;" width="100%"> <tbody style="break-inside: avoid !important; font-size: 12px; margin-top: 0px; line-height: 2.2; margin-bottom: 0px; text-align: justify;"> <tr style="break-inside: avoid !important;"> <td class="abstractmed" colspan="2" style="break-inside: avoid !important;"> <p style="font-size: 12px; margin-top: 0px; margin-bottom: 0px; text-align: center;"><span style="color: rgb(0, 0, 0); font-size: 12px; line-height: 20.8px;">لطفاً به چکیده انگلیسی مراجعه شود.</span></p> </td> </tr> <tr style="break-inside: avoid !important;"> </tr> </tbody> </table> <p></p> <p><strong>Introduction: </strong>Rosemary, <em>Rosmarinus (R.) officinalis</em> L. is a well-known plant with several useful properties such as analgesic, anti-inflammatory and anti-neurodegenerative. It has been used in folk medicine to alleviate rheumatic pain, stomachache and dysmenorrhea. Rosemary has several constituents such as rosmarinic acid which can be responsible for therapeutic properties been noted with rosemary. The aim of this study was to investigate the potential anti-inflammatory effects of <em>R. officinalis</em> and rosmarinic acid in a rat model of sciatic nerve chronic constriction injury (CCI)-induced neuropathic pain to verify usage of rosemary in folk medicine. <strong>Materials and Methods: </strong>Rats underwent CCI, were treated&nbsp; with either normal saline, ethanolic extract of aerial parts of <em>R<span dir="RTL">.</span> officinalis </em>(400 mg/kg, i.p.) or rosmarinic acid (40 mg/kg, i.p.) from the day of surgery (day 0) for 14 days. The anti-inflammatory effects of <em>R. officinalis</em> extract and rosmarinic acid were evaluated by assessing the levels of some spinal inflammatory markers including cyclooxygenase-2 (COX2), prostaglandin E2 (PGE-2), interleukin 1 beta (IL-1&beta;), matrix metallopeptidase 2 (MMP2) through western blotting and nitric oxide (NO) production via Griess reaction on days 7 and 14 post-surgery. <strong>Results</strong>: CCI rats exhibited a marked expression in the levels of inflammatory markers (COX2, PGE-2, IL-1&beta;, MMP2 and NO) on both days 7 (p < 0.001) and 14 (p < 0.001). Rosmarinic acid and ethanolic extract of <em>R. officinalis</em> were able to decrease amounts of mentioned inflammatory markers on both days 7 (p < 0.001) and 14 (p<0.001). <strong>Conclusion:</strong> Our data support the traditional use of <em>R. officinalis</em> as an effective remedy for pain relief and inflammatory disorders. It also suggests that the ethanolic extract of <em>R<span dir="RTL">.</span> officinalis</em> and rosmarinic acid through modulating neuro-inflammation might be potential candidates in treating neuropathic pain and different neurological disorders associated with inflammation.</p> Rosmarinus officinalis, Rosmarinic acid, Neuropathic pain, Chronic constriction injury, Inflammatory markers 64 64 http://shefayekhatam.ir/browse.php?a_code=A-10-24-882&slc_lang=en&sid=1 Mahboobeh Ghasemzadeh Rahbardar Mahboobeh Ghasemzadeh Rahbardar 100319475328460011910 100319475328460011910 No Department of Physiology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran Department of Physiology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran Bahareh Amin Bahareh Amin 100319475328460011911 100319475328460011911 No Cellular and Molecular Research Center, Department of Physiology and Pharmacology, Faculty of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran Cellular and Molecular Research Center, Department of Physiology and Pharmacology, Faculty of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran Hossein Hosseinzadeh Hossein Hosseinzadeh hosseinzadehh@mums.ac.ir 100319475328460011912 100319475328460011912 Yes Pharmaceutical Research Center, Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran Pharmaceutical Research Center, Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran