en P 43: The Role of TWIK2 Channels on Immune Cells and its Impact in EAE/ MS Pathophysiology تحقیقات پایه در علوم اعصاب Basic research in Neuroscience پژوهشي Research --- Open Access, CC-BY-NC <table border="0" cellpadding="5px" cellspacing="2" dir="rtl" id="table_article" style="font-family: Tahoma; background-color: rgb(251, 250, 249); break-inside: avoid !important;" width="100%"> <tbody style="break-inside: avoid !important; font-size: 12px; margin-top: 0px; line-height: 2.2; margin-bottom: 0px; text-align: justify;"> <tr style="break-inside: avoid !important;"> <td class="abstractmed" colspan="2" style="break-inside: avoid !important;"> <p style="font-size: 12px; margin-top: 0px; margin-bottom: 0px; text-align: center;"><span style="color: rgb(0, 0, 0); font-size: 12px;">لطفاً به چکیده انگلیسی مراجعه شود.</span></p> </td> </tr> <tr style="break-inside: avoid !important;"> </tr> </tbody> </table> <p></p> <p>TWIK2 ion channels (K_2P6.1, encoded by a gene named <em>Kcnk6</em>) belong to the family of two-pore domain potassium channels. TWIK2 is also considered as a &ldquo;silent&rdquo; channel because of its inability to produce measurable currents in heterologous expression systems and its intracellular retention. It is expressed in different mammalian tissues including lymphoid organs and the vascular system. However, the role that TWIK2 channels play in the immune system is far from being understood. Previous studies have already revealed that some other members of the K_2P channel family have a crucial role in the pathogenesis of multiple sclerosis (MS), an autoinflammatory disorder of the central nervous system. We aim at unravelling the reasons of TWIK2 channels being silent and their role on immune cells in respect to the pathophysiology of MS and its animal model experimental autoimmune encephalomyelitis (EAE) which might offer a novel therapeutic target. Hereby, we have investigated the distribution of TWIK2 channels in different tissues and cells, revealing strong TWIK2-expression levels on dendritic cells, macrophages and T-lymphocytes. Future experiments will focus on the role of TWIK2 channels <em>in vivo</em> by inducing MOG-specific EAE in TWIK2^-/- and WT animals to investigate the disease course and perform <em>ex vivo</em> functional assays with MOG-specific, autoreactive immune cells.</p> Mammalian, Immune Cells, Macrophages 74 74 http://shefayekhatam.ir/browse.php?a_code=A-10-24-892&slc_lang=en&sid=1 Li-Ming Lee Li-Ming Lee li-ming.lee@ukmuenster.de 100319475328460011945 100319475328460011945 Yes Department of Neurology, Clinic of Neurology and Institute for Translational Neurology, University of Münster, Münster, Germany Department of Neurology, Clinic of Neurology and Institute for Translational Neurology, University of Münster, Münster, Germany Juncal Fernandez-Orth Juncal Fernandez-Orth 100319475328460011946 100319475328460011946 No Department of Neurology, Clinic of Neurology and Institute for Translational Neurology, University of Münster, Münster, Germany Department of Neurology, Clinic of Neurology and Institute for Translational Neurology, University of Münster, Münster, Germany Tobias Ruck Tobias Ruck 100319475328460011947 100319475328460011947 No Department of Neurology, Clinic of Neurology and Institute for Translational Neurology, University of Münster, Münster, Germany Department of Neurology, Clinic of Neurology and Institute for Translational Neurology, University of Münster, Münster, Germany Stefan Bittner Stefan Bittner 100319475328460011948 100319475328460011948 No Department of Neurology, University Medical Center of the Johannes Gutenberg-University, Mainz, Germany Department of Neurology, University Medical Center of the Johannes Gutenberg-University, Mainz, Germany Nikole Bobak Nikole Bobak 100319475328460011949 100319475328460011949 No LabEx ICST, Institut de Pharmacologie Moléculaire et Cellulaire, CNRS, and Université de Nice Sophia-Antipolis, Sophia-Antipolis, Valbonne, France LabEx ICST, Institut de Pharmacologie Moléculaire et Cellulaire, CNRS, and Université de Nice Sophia-Antipolis, Sophia-Antipolis, Valbonne, France Florian Lesage Florian Lesage 100319475328460011950 100319475328460011950 No LabEx ICST, Institut de Pharmacologie Moléculaire et Cellulaire, CNRS, and Université de Nice Sophia-Antipolis, Sophia-Antipolis, Valbonne, France LabEx ICST, Institut de Pharmacologie Moléculaire et Cellulaire, CNRS, and Université de Nice Sophia-Antipolis, Sophia-Antipolis, Valbonne, France Sven Meuth Sven Meuth 100319475328460011951 100319475328460011951 No Department of Neurology, Clinic of Neurology and Institute for Translational Neurology, University of Münster, Münster, Germany Department of Neurology, Clinic of Neurology and Institute for Translational Neurology, University of Münster, Münster, Germany