en P30: Effects of Hemin on Ca2+Influx in Neurons of C57BL/6 Mouse Brain تحقیقات پایه در علوم اعصاب Basic research in Neuroscience پژوهشي Research --- Open Access, CC-BY-NC <span style="text-align: center; color: rgb(0, 0, 0); text-transform: none; text-indent: 0px; letter-spacing: normal; font-family: Tahoma; font-size: 12px; font-style: normal; font-weight: 400; word-spacing: 0px; float: none; display: inline !important; white-space: normal; orphans: 2; widows: 2; background-color: rgb(251, 250, 249); font-variant-ligatures: normal; font-variant-caps: normal; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial;">لطفاً به چکیده انگلیسی مراجعه شود.</span> <div style="text-align: justify;">Excitotoxicity results in a significant increase in Ca2+ influx; essentially from open N-Methyl-D-aspartate receptors (NMDARs) channels that cause a secondary rise in the intracellular Ca2+ concentration. It is correlated with neuronal death induced by Ca2+ overload. Dysfunction of NMDARs is associated with excitotoxic neuronal death in neurodegenerative disorders. In this study, the effects of hemin on Ca2+ permeability in neurons of C57BL/6 mouse brain examined. Isolated from 1-dayold C57BL/6 mice, were cultured in serum-free media. Cells were maintained in growth medium at 37◦C in 95% air/5% CO2 for 2 weeks in vitro before treatment. Primary neurons were cultured in serum-free media were treated with hemin (0, 12.5, 25, 50, 75,100 &mu;M) for 18 (h). Intensity of calcium fluorescence was reduced in treated cultures with hemin (100, 86, 78.5, 60, 56, 46%, respective to the concentrations stated previously; P<0.05 for all). Hemin increased Ca2+ influx in cultured neurons. NMDAR stimulation by hemin increased the activating of NMDARs and Ca2+ influx in the cultured neurons. Therefore, heminis cytotoxic due to increase of intracellular Ca2+ influx.<br> &nbsp;</div> Mouse, Hemin, Neurons, Treatment 61 61 http://shefayekhatam.ir/browse.php?a_code=A-10-24-1166&slc_lang=en&sid=1 Hamid Islampoor Hamid Islampoor bidgani777@yahoo.com 100319475328460015556 100319475328460015556 Yes Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran