fa O36: Three-Dimensional Transplantation of Adult Neural Stem Cells in an Acute Brain Injury Model تحقیقات پایه در علوم اعصاب Basic research in Neuroscience پژوهشي Research --- Open Access, CC-BY-NC <p align="center">لطفاً به چکیده انگلیسی مراجعه شود. </p> Brain injury is the leading cause of death and disability in the most active population. There is no definite clinical treatment for repair of damaged brain tissue. Application of nanoscaffolds supporting stem cells can be helpful. The present study examined the effects of autologous adult neural stem/progenitor cells (NS/PCs) seeded in PuraMatrix in acute brain injury. The right brain subventricular zone of adult wistar rats was stereotactically harvested. Then, NS/PCs were cultured using neurosphere assay. At day 45, brain injury was performed in left side of brain and PBS, PuraMatrix, NS/PCs or PuraMatrix+ NS/PCs was injected into the created cavity. The neurological status was evaluated for 4 weeks. Then, morphological and immunohistochemical studies were done. The neurologic status improved after treatment of brain injury with PuraMatrix, NS/PCs or PuraMatrix + NS/PCs. The lesion volume was decreased in PuraMatrix+ NS/PCs. By 3D transplantation of NS/PCs, not only the rate of inflammation was reduced but also the survival rate in the site of injury was increased. In addition, the transplanted cells expressed the differentiation markers after 4 weeks. Transplantation of adult NS/PCs in PuraMatrix may be a feasible method for reduction of tissue damage following brain injury. Brain Injury, Neural Stem Cells, Autologous Transplantation, Nanoscaffold, Neural Tissue Engineering 36 36 http://shefayekhatam.ir/browse.php?a_code=A-10-24-381&slc_lang=fa&sid=1 Hadi Aligholi Hadi Aligholi 10031947532846005580 10031947532846005580 No a. Shefa Neuroscience Research Center, Khatam- al- Anbia Hospital, Tehran, Iran. b. Department of Neuroscience, School of Advanced Technologies in medicine, Tehran University of Medical Sciences, Tehran, Iran. a. Shefa Neuroscience Research Center, Khatam- al- Anbia Hospital, Tehran, Iran. b. Department of Neuroscience, School of Advanced Technologies in medicine, Tehran University of Medical Sciences, Tehran, Iran. Gholamreza Hassanzadeh Gholamreza Hassanzadeh hassanzadeh@tums.ac.ir 10031947532846005581 10031947532846005581 Yes Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Mahdi Rezayat Mahdi Rezayat 10031947532846005582 10031947532846005582 No Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Hassan Azari Hassan Azari 10031947532846005583 10031947532846005583 No a. Neural Stem Cell & Regenerative Neuroscience Laboratory, Department of Anatomical Sciences, School of Medicine, Shiraz, Iran. b. Shiraz Stem Cell Institute, Shiraz University of Medical Sciences, Shiraz, Iran. a. Neural Stem Cell & Regenerative Neuroscience Laboratory, Department of Anatomical Sciences, School of Medicine, Shiraz, Iran. b. Shiraz Stem Cell Institute, Shiraz University of Medical Sciences, Shiraz, Iran. Shahram Ejtemaei Mehr Shahram Ejtemaei Mehr 10031947532846005584 10031947532846005584 No Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Mohammad Akbari Mohammad Akbari 10031947532846005585 10031947532846005585 No Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Mostafa Modarres Mousavi Mostafa Modarres Mousavi 10031947532846005586 10031947532846005586 No Shefa Neuroscience Research Center, Khatam- al- Anbia Hospital, Tehran, Iran. Shefa Neuroscience Research Center, Khatam- al- Anbia Hospital, Tehran, Iran. Fatemeh Alipour Fatemeh Alipour 10031947532846005587 10031947532846005587 No Shefa Neuroscience Research Center, Khatam- al- Anbia Hospital, Tehran, Iran. Shefa Neuroscience Research Center, Khatam- al- Anbia Hospital, Tehran, Iran. Ali Gorji Ali Gorji 10031947532846005588 10031947532846005588 No a. Epilepsy Research Center and b. Department of neurosurgery and neurology, Westfälische Wilhelms-Universität Münster, Robert-Koch-Strasse 27a, 48149 Münster, Germany. a. Epilepsy Research Center and b. Department of neurosurgery and neurology, Westfälische Wilhelms-Universität Münster, Robert-Koch-Strasse 27a, 48149 Münster, Germany.