fa W6: Self‐Assembling Peptide Nanofiber Containing Biologic Motif Induces Neural Differentiation, Tubulin Polymerization and Neurogenesis In‐Vitro, Ex‐Vivo and In‐Vivo Studies تحقیقات پایه در علوم اعصاب Basic research in Neuroscience پژوهشي Research --- Open Access, CC-BY-NC <p align="center">لطفاً به چکیده انگلیسی مراجعه شود. </p> Spinal cord injury (SCI) in humans remains a devastating and incurable disorder. A very important obstacle in axonal regeneration after spinal cord injury is astroglial scaring. The use of self-assembling peptide nanofiber, a hydrogel mimicking extracellular matrix, has been suggested as a scaffold for spinal cord regeneration and inhibition of astrogliosis. However, neurogenesis potential of laminim has been proved. The purpose of this study was to investigate the role of self-assembling peptide nanofiber containing long motif of laminin (SAPN-LL) in neural differentiation of human endometrial-derived stem cells (hEnSCs) in-vitro, in polymerization of tubulin isolated from sheep brain ex-vivo and assess the supportive effects of this hydrogel in an animal model of SCI. Results showed that although nanaofibers strongly differentiated hEnSC towards neuron and there were not significant differences between their neural differentiations but motor recovery results demonstrated that concentration of laminin influences motor recovery and tubulin polymerization. However, both of in-vitro and in-vivo results showed that SAPN-LL inhibited astrogenesis. Based on our results it might be concluded that, SAPN containing long motif of laminin holds great promise for spinal cord injury. Self-assembling Peptide Nanofiber, Long Motif of Laminin, Spinal Cord Injury, Polymerization of Tubulin 49 49 http://shefayekhatam.ir/browse.php?a_code=A-10-24-395&slc_lang=fa&sid=1 Shima Tavakol Shima Tavakol 10031947532846005617 10031947532846005617 No a. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran. b. Razi Drug Research Center, Iran University of Medical Sciences, Tehran, Iran. c. Student’s Scientific Research Center, Tehran University of Medical Sciences, Tehran, Iran. d. Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran. a. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran. b. Razi Drug Research Center, Iran University of Medical Sciences, Tehran, Iran. c. Student’s Scientific Research Center, Tehran University of Medical Sciences, Tehran, Iran. d. Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran. Reza Saber Reza Saber 10031947532846005618 10031947532846005618 No Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran. Elham Hoveizi Elham Hoveizi 10031947532846005619 10031947532846005619 No Department of Biology, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran. Department of Biology, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran. Hadi Aligholi Hadi Aligholi 10031947532846005620 10031947532846005620 No a. Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran. b. Department of Neurosciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran. a. Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran. b. Department of Neurosciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran. Jafar Ai Jafar Ai 10031947532846005621 10031947532846005621 No a. Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran. b. Brain and Spinal Injury Research Center, Imam Hospital, Tehran University of Medical Sciences, Tehran, Iran. a. Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran. b. Brain and Spinal Injury Research Center, Imam Hospital, Tehran University of Medical Sciences, Tehran, Iran. Mahdi Rezayat Mahdi Rezayat Rezayat@tums.ac.ir 10031947532846005622 10031947532846005622 Yes a. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran. b. Department of Toxicology & Pharmacology, School of Pharmacy, Pharmaceutical Sciences Branch, Islamic Azad University (IAUPS), Tehran, Iran. c. Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. a. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran. b. Department of Toxicology & Pharmacology, School of Pharmacy, Pharmaceutical Sciences Branch, Islamic Azad University (IAUPS), Tehran, Iran. c. Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.