fa P6: Neuroprotective Effect of Estrogen against Brain Edema and Blood Brain Barrier Disruption: Roles of Estrogen Receptors α and β تحقیقات پایه در علوم اعصاب Basic research in Neuroscience پژوهشي Research --- Open Access, CC-BY-NC <p align="center">لطفاً به چکیده انگلیسی مراجعه شود. </p> Estrogen (E2) has neuroprotective effects on blood-brain-barrier (BBB) after traumatic brain injury (TBI). In order to investigate the roles of estrogen receptors (ERs) in these kind of effects, ER-α antagonist (MPP) and, ER-β antagonist (PHTPP), or non-selective estrogen receptors antagonist (ICI 182780) were administered as regulators of CNS cytokines levels and neuroinflammation after TBI. MPP (150 µg/Kg), PHTPP (150 µg/Kg) or ICI182780 (4 mg/kg) was injecteddaily 48hr before TBI, then E2 (33.3µg/Kg) or oil were administered 30 min after TBI. BBB disruption (Evans blue content) and brain edema (brain water content) were evaluated 5hr and 24hr after the TBI, respectively. Brain levels of anti-inflammatory (IL-10 and IL-1ra) and proinflammatory (IL-1β, IL-6, and TNF-α) cytokines were quantified 24hr after TBI induced by Marmarou’s method. Results revealed that, in the presence of each selective estrogen receptor antagonist there was a significant increase ofIL-10and significant decrease of IL-1β, IL-6, and TNF-α 24hr after TBI but there is no significant differences between the results of combined use of selective receptor antagonists and the non-selective one. Taken together, these studies identified a dramatic cytokine-mediated neuroinflammatory response that is regulated through both ER-α and ER-β receptors. This may suggest a therapeutic potential in the brain trauma for ER-specific agonists. ER-α Agonist, ER-β Agonist, Cytokines, Neuroinflammation, Estrogen 56 56 http://shefayekhatam.ir/browse.php?a_code=A-10-24-402&slc_lang=fa&sid=1 Vida Naderi Vida Naderi vdnaderi@gmail.com 10031947532846005651 10031947532846005651 Yes Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, Iran. Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, Iran. Mohammad Khaksari Mohammad Khaksari 10031947532846005652 10031947532846005652 No Physiology Research Center, Kerman University of Medical Sciences, Kerman, Iran. Physiology Research Center, Kerman University of Medical Sciences, Kerman, Iran. Fatemeh Maghool Fatemeh Maghool 10031947532846005653 10031947532846005653 No Physiology Research Center, Kerman University of Medical Sciences, Kerman, Iran. Physiology Research Center, Kerman University of Medical Sciences, Kerman, Iran.