fa P12: Cultivation of Neural Stem-Like Cells from Adult Human Amygdala تحقیقات پایه در علوم اعصاب Basic research in Neuroscience پژوهشي Research --- Open Access, CC-BY-NC <p align="center">لطفاً به چکیده انگلیسی مراجعه شود. </p> Several sources were introduced for cell therapy after neurotrauma. There are some evidences that quiescent stem cells are located in the various regions of the adult mammalian brain including cortex, hippocampus, amygdala and striatum. But more study is needed to identify the characteristics of these cells. In the present study we investigated the possibility of isolating neural stem cells from adult human amygdala. The amygdala specimens were obtained from five patients suffering from refractory temporal lobe epilepsy and subjected to amygdalo-hippocampectomy. After removing the pia mater and associated blood vessels, the tissue was dissociated enzymatically. Then, the single cells were cultured in neurosphere medium containing 20 ng/ml Fibroblast growth factor, 20 ng/ml epidermal growth factor, B27 supplement and N2 supplement in non-coated flasks. Growth factors were added twice a week. Additional neurosphere medium was administered once every week. To characterize the isolated cells, immunocytochemistry was done against nestin, Sox2, Oct4, GFAP and MAP2. The isolated cells highly expressed neural stem cell markers nestin, Sox2 and Oct4. But there was a few cells expressed mature neuron marker MAP2 and astrocyte marker GFAP. Here, we showed for the first time in Iran the possibility of isolating neural stem-like cells from patients with refrac‌tory epilepsy during interventional surgery. Amygdala, Human, Neural Stem-Like Cells, Epilepsy 62 62 http://shefayekhatam.ir/browse.php?a_code=A-10-24-408&slc_lang=fa&sid=1 Hadi Aligholi Hadi Aligholi aligholihadi@gmail.com 10031947532846005672 10031947532846005672 Yes Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran. Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran. Sedigheh Ghasemi Sedigheh Ghasemi 10031947532846005673 10031947532846005673 No Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran. Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran. Sajad Sahab Negah Sajad Sahab Negah 10031947532846005674 10031947532846005674 No Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran. Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran. Mostafa Modarres Mousavi Mostafa Modarres Mousavi 10031947532846005675 10031947532846005675 No Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran. Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran. Ali Gorji Ali Gorji 10031947532846005676 10031947532846005676 No Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran. Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran.