The Neuroscience Journal of Shefaye Khatam
مجله علوم اعصاب شفای خاتم
Shefaye Khatam
Medical Sciences
http://shefayekhatam.ir
1
admin
2322-1887
2345-4814
10.61186/shefa
fa
jalali
1393
9
1
gregorian
2014
12
1
2
4
online
1
fulltext
fa
P41: Therapeutic Effect of Transplanting Neural Stem Cells Derived Bone Marrow Stromal Cells Using Bioactive Substance TNT, for Enhance Recovery from Spinal Cord Injury
P41: Therapeutic Effect of Transplanting Neural Stem Cells Derived Bone Marrow Stromal Cells Using Bioactive Substance TNT, for Enhance Recovery from Spinal Cord Injury
تحقیقات پایه در علوم اعصاب
Basic research in Neuroscience
پژوهشي
Research --- Open Access, CC-BY-NC
<p align="center">لطفاً به چکیده انگلیسی مراجعه شود. </p>
Bone marrow mesenchymal stem cells can differentiate into neurons and astrocytes after transplantation in the spinal cord injury of rats. Although bone marrow mesenchymal stem cells are known to protect against spinal cord injury through anti-apoptotic effects, but generation of neural stem cells with large number and safety protocols unclear. In the present study, described the differentiation of bone marrow stromal cells (BMSC) into a neural stem cell-like under the influence of a factor inducing non-toxic bioactive substance TNT.In the present study, bone marrow mesenchymal stem cells were cultured and proliferated, then induced with bioactive substance TNT. That these cells differentiate as a neurosphere-like structures and neural stem cells. These cells approved by immunocytochemistry and RT-PCR technique. These cells transplanted into rats with spinal cord injury. Immunohistochemistry and immunofluorescence with subsequent quantification revealed that the expression of the axonal regeneration marker and the neuronal marker, microtubule-associated protein 2, significantly increased in rats with bone marrow mesenchymal stem cell transplantation compared with those in rats with spinal cord injury. Our results suggest that bone marrow mesenchymal stem cell transplantation promotes neurite growth and regeneration and prevents autophagy. These responses may likely be mechanisms underlying the protective effect of bone marrow mesenchymal stem cells against spinal cord injury. Transplanting BMSCs can enhance the protein expression of neural growth factor in the rats which undergo injury to their spinal cord. It can be significantly improved the rehabilitation of the motor function. The improvement is associated with the transplantation of BMSCs which are beneficial for regeneration and repair of the rat’s spinal cord injury.
BMSC, Spinal Cord Injury, Transplantation, Neural Stem Cells
91
91
http://shefayekhatam.ir/browse.php?a_code=A-10-24-437&slc_lang=fa&sid=1
Parastoo
Barati
Parastoo
Barati
10031947532846005823
10031947532846005823
No
Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran.
Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran.
Alireza
Moradi
Alireza
Moradi
10031947532846005824
10031947532846005824
No
Department of Physiology, Islamic Azad University of Qom, Qom, Iran.
Department of Physiology, Islamic Azad University of Qom, Qom, Iran.
Marzieh
Darvishi
Marzieh
Darvishi
Marzidarvish@yahoo.com
10031947532846005825
10031947532846005825
Yes
Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran.
Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran.
Taghi
Tiraihi
Taghi
Tiraihi
10031947532846005826
10031947532846005826
No
Department of Anatomy, Tarbiat Modares University, Tehran, Iran.
Department of Anatomy, Tarbiat Modares University, Tehran, Iran.