fa P77: Cell Therapy Approaches to Enhancing Neuro-Regeneration after Spinal Cord Injury: Generation Neural Stem Cells from Neurosphere-Derived Adipose Stem Cells Using Bioactive Substance TNT تحقیقات پایه در علوم اعصاب Basic research in Neuroscience پژوهشي Research --- Open Access, CC-BY-NC <p align="center">لطفاً به چکیده انگلیسی مراجعه شود. </p> In the last few years, a lot of preclinical studies showed the therapeutic potential of stem cells in spinal cord injury (SCI). Neural stem cells (NSCs) can be differentiated into all cell types of spinal cord. NSCs are cells that maintain the capacity to differentiate into brain-specific cell types, and may also replace or repair of injury in central nervous system. Here in, we describe the efficient conversion of Adipose derived stem cells (ADSCs) into a neural stem cell-like under the influence of a factor inducing non-toxic bioactive substance TNT. ADSCs were isolated from adipose tissue of Wistar rat and were cultured. ADSCs were treated with bioactive substance TNT. These cells grew in neurosphere-like structures and differentiated to neural stem cells. Immunocytochemistry and RT-PCR techniques were performed to evaluate early neuroectodermal markers including SOX2, OCT4, NANOG, NeuroD and nestin. Our results showed that these markers expressed in high level. Also, NSCs were immunoreactive to NF68, NF200 and nestin. In addition, we confirmed the expression of SOX2, OCT4, NANOG and NeuroD genes by RT-PCR assay. The findings of this study provide a new method to generate NSCs from ADSCs by using non-toxic bioactive substance TNT, which can be helpful in cell therapy of SCI and degenerative diseases. Neuroregeneration, Cell Therapy, Neurodegenerative Disease 127 127 http://shefayekhatam.ir/browse.php?a_code=A-10-24-475&slc_lang=fa&sid=1 Prastoo Barati Prastoo Barati 10031947532846005968 10031947532846005968 No Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran. Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran. Taghi Tiraihi Taghi Tiraihi ttiraihi@yahoo.com 10031947532846005969 10031947532846005969 Yes a. Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran. b. Department of Anatomy, Tarbiat Modares University, Tehran, Iran. a. Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran. b. Department of Anatomy, Tarbiat Modares University, Tehran, Iran. Marzieh Darvishi Marzieh Darvishi 10031947532846005970 10031947532846005970 No a. Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran. b. Department of Anatomy, Tarbiat Modares University, Tehran, Iran. a. Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran. b. Department of Anatomy, Tarbiat Modares University, Tehran, Iran. Hadi Kazemi Hadi Kazemi 10031947532846005971 10031947532846005971 No Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran. Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran.