The Neuroscience Journal of Shefaye Khatam
مجله علوم اعصاب شفای خاتم
Shefaye Khatam
Medical Sciences
http://shefayekhatam.ir
1
admin
2322-1887
2345-4814
10.61186/shefa
fa
jalali
1393
9
1
gregorian
2014
12
1
2
4
online
1
fulltext
fa
P77: Cell Therapy Approaches to Enhancing Neuro-Regeneration after Spinal Cord Injury: Generation Neural Stem Cells from Neurosphere-Derived Adipose Stem Cells Using Bioactive Substance TNT
P77: Cell Therapy Approaches to Enhancing Neuro-Regeneration after Spinal Cord Injury: Generation Neural Stem Cells from Neurosphere-Derived Adipose Stem Cells Using Bioactive Substance TNT
تحقیقات پایه در علوم اعصاب
Basic research in Neuroscience
پژوهشي
Research --- Open Access, CC-BY-NC
<p align="center">لطفاً به چکیده انگلیسی مراجعه شود. </p>
In the last few years, a lot of preclinical studies showed the therapeutic potential of stem cells in spinal cord injury (SCI). Neural stem cells (NSCs) can be differentiated into all cell types of spinal cord. NSCs are cells that maintain the capacity to differentiate into brain-specific cell types, and may also replace or repair of injury in central nervous system. Here in, we describe the efficient conversion of Adipose derived stem cells (ADSCs) into a neural stem cell-like under the influence of a factor inducing non-toxic bioactive substance TNT. ADSCs were isolated from adipose tissue of Wistar rat and were cultured. ADSCs were treated with bioactive substance TNT. These cells grew in neurosphere-like structures and differentiated to neural stem cells. Immunocytochemistry and RT-PCR techniques were performed to evaluate early neuroectodermal markers including SOX2, OCT4, NANOG, NeuroD and nestin. Our results showed that these markers expressed in high level. Also, NSCs were immunoreactive to NF68, NF200 and nestin. In addition, we confirmed the expression of SOX2, OCT4, NANOG and NeuroD genes by RT-PCR assay. The findings of this study provide a new method to generate NSCs from ADSCs by using non-toxic bioactive substance TNT, which can be helpful in cell therapy of SCI and degenerative diseases.
Neuroregeneration, Cell Therapy, Neurodegenerative Disease
127
127
http://shefayekhatam.ir/browse.php?a_code=A-10-24-475&slc_lang=fa&sid=1
Prastoo
Barati
Prastoo
Barati
10031947532846005968
10031947532846005968
No
Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran.
Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran.
Taghi
Tiraihi
Taghi
Tiraihi
ttiraihi@yahoo.com
10031947532846005969
10031947532846005969
Yes
a. Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran. b. Department of Anatomy, Tarbiat Modares University, Tehran, Iran.
a. Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran. b. Department of Anatomy, Tarbiat Modares University, Tehran, Iran.
Marzieh
Darvishi
Marzieh
Darvishi
10031947532846005970
10031947532846005970
No
a. Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran. b. Department of Anatomy, Tarbiat Modares University, Tehran, Iran.
a. Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran. b. Department of Anatomy, Tarbiat Modares University, Tehran, Iran.
Hadi
Kazemi
Hadi
Kazemi
10031947532846005971
10031947532846005971
No
Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran.
Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran.