:: Volume 9, Issue 3 (Summer 2021) ::
Shefaye Khatam 2021, 9(3): 55-63 Back to browse issues page
Synthesis, Characterization, Evaluation of Supportive Properties, and Neuroprotective Effects of Cerium Oxide Nanoparticles as a Candidate for Neural Tissue Engineering
Yasaman Arzanipur , Arash Abdolmaleki * , Asadollah Asadi , Saber Zahri
a. Department of Engineering Sciences, Faculty of Advanced Technologies, University of Mohaghegh Ardabili, Namin, Iran. b. Bio Science and Biotechnology Research center (BBRC), Sabalan University of Advanced Technologies (SUAT), Namin, Iran , abdolmalekiarash1364@gmail.com
Abstract:   (2118 Views)
Introduction: Tissue engineering is a part of biotechnology that includes the development of biological implants for tissue regeneration to improve tissue or organ function. This study aimed to investigate the effect of cerium oxide nanoparticles on the interactions between adipose tissue stem cells and decellularized sciatic nerve scaffolds in rats. Materials and Methods: Rats were anesthetized by injecting a mixture of ketamine (80 mg/kg) and xylazine (10 mg/kg). Sciatic nerve fragments (15 mm) were removed above the three-pronged site in the thigh muscle and decellularized after cleaning the surrounding tissues using the Sandal method. Then, adipose tissue mesenchymal cells were implanted on the scaffold, and the growth and viability of the cells implanted on the scaffold in the presence of cerium oxide nanoparticles were measured by MTT assay. Results: The results of histological evaluations showed that the scaffolds were completely decellularized and hematoxylin/eosin and Dapi staining confirmed these results. Specialized tissue evaluation by Masson trichrome staining as well as biomechanical analysis showed that collagen and elastin fibers were relatively preserved in the extracellular matrix. Cell viability on the scaffold increased in the presence of nanoparticles. Conclusion: Cerium oxide nanoparticles increase cell stability, proliferation, and maintenance of adipose tissue mesenchymal cells and may be beneficial in the treatment of peripheral nerve lesions.
Keywords: Sciatic Nerve, Cerium, Extracellular Matrix, Regeneration, Tissue Engineering
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Type of Study: Research --- Open Access, CC-BY-NC | Subject: Neural Repair
1. Abbaszadeh S, Asadi A, Zahri S, Abdolmaleki A, Mahmoudi F. Does Phenytoin Have Neuroprotective Role and Affect Biocompatibility of Decellularized Sciatic Nerve Scaffold? Gene, Cell and Tissue. 2020; (In Press). [DOI:10.5812/gct.108726]
2. Abdolmaleki A, Zahri S, Bayrami A. Rosuvastatin enhanced functional recovery after sciatic nerve injury in the rat. European Journal of Pharmacology. 2020: 173260. [DOI:10.1016/j.ejphar.2020.173260]
3. Asadi A, Zahri S, Abdolmaleki A. Biosynthesis, characterization and evaluation of the supportive properties and biocompatibility of DBM nanoparticles on a tissue-engineered nerve conduit from decellularized sciatic nerve. Regenerative Therapy. 2020; 14: 315-21. [DOI:10.1016/j.reth.2020.03.004]
4. Soluki M, Mahmoudi F, Abdolmaleki A, Asadi A, Namini AS. Cerium oxide nanoparticles as a new neuroprotective agent to promote functional recovery in a rat model of sciatic nerve crush injury. British Journal of Neurosurgery. 2020. [DOI:10.1080/02688697.2020.1864292]
5. Sarkar A, Saha S, Paul A, Maji A, Roy P, Maity TK. Understanding stem cells and its pivotal role in regenerative medicine. Life sciences. 119270: 2021. [DOI:10.1016/j.lfs.2021.119270]
6. Abdolmaleki A, Zahri S, Asadi A, Wassersug R. Role of Tissue Engineering and Regenerative Medicine in Treatment of Sport Injuries. Trauma Monthly. 2020; 25(3): 106-12.
7. Abdolmaleki A, Asadi A, Taghizadeh Momen L, Parsi Pilerood S. The Role of Neural Tissue Engineering in the Repair of Nerve Lesions. The Neuroscience Journal of Shefaye Khatam. 2020; 8(3): 80-96. [DOI:10.29252/shefa.8.3.80]
8. Ghayour MB, Abdolmaleki A, Fereidoni M. Use of stem cells in the regeneration of peripheral nerve injuries: an overview. The Neuroscience Journal of Shefaye Khatam. 2015; 3(1): 84-98. [DOI:10.18869/acadpub.shefa.3.1.84]
9. Sowa Y, Imura T, Numajiri T, Nishino K, Fushiki S. Adipose-derived stem cells produce factors enhancing peripheral nerve regeneration: influence of age and anatomic site of origin. Stem cells and development. 2012; 21(11): 1852-62. [DOI:10.1089/scd.2011.0403]
10. Carlson KB, Singh P, Feaster MM, Ramnarain A, Pavlides C, Chen ZL, et al. Mesenchymal stem cells facilitate axon sorting, myelination, and functional recovery in paralyzed mice deficient in Schwann cell‐derived laminin. Glia. 2011; 59(2): 267-77. [DOI:10.1002/glia.21099]
11. Marconi S, Castiglione G, Turano E, Bissolotti G, Angiari S, Farinazzo A, et al. Human adipose-derived mesenchymal stem cells systemically injected promote peripheral nerve regeneration in the mouse model of sciatic crush. Tissue Engineering Part A. 2012; 18(11-12): 1264-72. [DOI:10.1089/ten.tea.2011.0491]
12. Gayour MB, Abdolmaleki A, Fereidoni M. Role of extracellular matrix in peripheral nerve regeneration process. 2015.
13. Abdolmaleki A, Ghayour M-B, Zahri S, Asadi A, Behnam-Rassouli M. Preparation of acellular sciatic nerve scaffold and it's mechanical and histological properties for use in peripheral nerve regeneration. Tehran University Medical Journal TUMS Publications. 2019; 77(2): 115-22.
14. Najafi R, Hosseini A, Ghaznavi H, Mehrzadi S, Sharifi AM. Neuroprotective effect of cerium oxide nanoparticles in a rat model of experimental diabetic neuropathy. Brain Research Bulletin. 2017; 131: 117-22. [DOI:10.1016/j.brainresbull.2017.03.013]
15. Polak P, Shefi O. Nanometric agents in the service of neuroscience: manipulation of neuronal growth and activity using nanoparticles. Nanomedicine: Nanotechnology, Biology and Medicine. 2015; 11(6): 1467-79. [DOI:10.1016/j.nano.2015.03.005]
16. Campbell GR, Campbell JH. Development of tissue engineered vascular grafts. Current pharmaceutical biotechnology. 2007; 8(1): 43-50. [DOI:10.2174/138920107779941426]
17. Ghayour M-B, Abdolmaleki A, Behnam-Rassouli M, Mahdavi-Shahri N, Moghimi A. Synergistic effects of Acetyl-L-carnitine and adipose-derived stromal cells to improving regenerative capacity of acellular nerve allograft in sciatic nerve defect. Journal of Pharmacology and Experimental Therapeutics. 2019: jpet. 118.254540.
18. Zuo KJ, Shafa G, Chan K, Zhang J, Hawkins C, Tajdaran K, et al. Local FK506 drug delivery enhances nerve regeneration through fresh, unprocessed peripheral nerve allografts. Experimental Neurology. 2021: 113680. [DOI:10.1016/j.expneurol.2021.113680]
19. Lajevardi M, Behnam-Rassouli M, Mahdavi-Shahri N, Abdolmaleki A, Mahdizadeh AH. Biocompatibility of Blastema Cells Derived from Rabbit's Pinna on Chitosan/Gelatin Micro-Nanofiber Scaffolds. Zahedan Journal of Research in Medical Sciences. 2019; 21(3). [DOI:10.5812/zjrms.11013]
20. Rajangam T, An SSA. Fibrinogen and fibrin based micro and nano scaffolds incorporated with drugs, proteins, cells and genes for therapeutic biomedical applications. International journal of nanomedicine. 2013; 8: 364.
21. Wang Y, Zhao Z, Ren Z, Zhao B, Zhang L, Chen J, et al. Recellularized nerve allografts with differentiated mesenchymal stem cells promote peripheral nerve regeneration. Neuroscience letters. 2012; 514(1): 96-101. [DOI:10.1016/j.neulet.2012.02.066]
22. Moore AM, MacEwan M, Santosa KB, Chenard KE, Ray WZ, Hunter DA, et al. Acellular nerve allografts in peripheral nerve regeneration: a comparative study. Muscle & nerve. 2011; 44(2): 221-34. [DOI:10.1002/mus.22033]
23. Wang W, Itoh S, Matsuda A, Ichinose S, Shinomiya K, Hata Y, et al. Influences of mechanical properties and permeability on chitosan nano/microfiber mesh tubes as a scaffold for nerve regeneration. Journal of biomedical materials research Part A. 2008; 84(2): 557-66. [DOI:10.1002/jbm.a.31536]
24. Kim CK, Kim T, Choi IY, Soh M, Kim D, Kim YJ, et al. Ceria nanoparticles that can protect against ischemic stroke. Angewandte Chemie International Edition. 2012; 51(44): 11039-43. [DOI:10.1002/anie.201203780]
25. Nelson BC, Johnson ME, Walker ML, Riley KR, Sims CM. Antioxidant cerium oxide nanoparticles in biology and medicine. Antioxidants. 2016; 5(2): 15. [DOI:10.3390/antiox5020015]
26. Eitan E, Hutchison ER, Greig NH, Tweedie D, Celik H, Ghosh S, et al. Combination therapy with lenalidomide and nanoceria ameliorates CNS autoimmunity. Experimental neurology. 2015; 273: 151-60. [DOI:10.1016/j.expneurol.2015.08.008]
27. D'Angelo B, Santucci S, Benedetti E, Di Loreto S, Phani R, Falone S, et al. Cerium oxide nanoparticles trigger neuronal survival in a human Alzheimer disease model by modulating BDNF pathway. Current Nanoscience. 2009; 5(2): 167-76. [DOI:10.2174/157341309788185523]
28. Das M, Patil S, Bhargava N, Kang J-F, Riedel LM, Seal S, et al. Auto-catalytic ceria nanoparticles offer neuroprotection to adult rat spinal cord neurons. Biomaterials. 2007; 28(10): 1918-25. [DOI:10.1016/j.biomaterials.2006.11.036]

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