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Showing 3 results for Zhiani
Sahar Babaei Abrak, Rahele Zhiani, Volume 2, Issue 1 (Winter - 2014)
Abstract
Introduction: Lemon herbena (Lippia citriodora) is a member of Verbenaceae family. This plant is endemic to South America and can growth in other areas, such as Iran. The aim of this study was to investigate and compare the chemical composition of the two production plants in the greenhouse and field of a lemon. Materials and Methods: Plants were cultivated in a Sarayan Ferdowsi area (south of Khorasan). Fourth kg of leaves was collected. Then, essential oil was extracted from fresh leaves using water and steam distillation. Finally, we treated PC12 cells with the essential oil that obtained from Lippia citriodora. The cell viability was evaluated by the MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide) assay. Results: Our results from GC-MS (Gas chromatography–mass spectrometry) method showed that greenhouse and field samples contained 0.68% and 0.62% (w/w), respectively. In addition, the data showed that the essential oil decreased oxidative stress-induced PC12 cell death. Conclusion: The results suggest that Lippia citriodora could be a potential candidate for treatment of neurodegenerative diseases.
Shokufe Emrani, Rahele Zhiani, Samane Dolatabadi, Volume 3, Issue 3 (Summer - 2015)
Abstract
Introduction: Hymenocrater is an important genus of Lamiaceae family. Hymenocrater platystegius is one of species in this genus and this plant is endemic to Iran and it grows wildly in the north east of Iran. The aim of this study was to investigate the chemical composition of Hymenocrater platystegius and to study the neuroprotective effect of essential oil of this plant in H2O2 –induced neurotoxicity in PC12 cells. Materials and Methods: The essential oil was extracted by hydrodistilation of air-dried sample for 5 hours. The essential oil composition of the aerial parts of Hymenocrater platystegius was studied by gas chromatography mass spectrometry. Finally, we treated PC12 cells with the essential oil of Hymenocrater platystegius. Results: The yield of essential oil was 0.1 % (w/w). Fifty-nine compounds were identified representing about 84.72% of the total oil. The major components of this oil were 1, 8-cineole (14.27%), ß-pinene (4.89%), Terpinolene (4.83%), and Sabinene (4.59%). In addition, the data showed that the essential oil decreased oxidative stress-induced PC12 cells death. Conclusion: The results suggest that Hymenocrater platystegius could be a potential candidate for treatment of neurodegenerative diseases.
Shokufe Emrani, Rahele Zhiani, Samane Dolatabadi, Volume 3, Issue 3 (Summer - 2015)
Abstract
Introduction: Excessive accumulation of beta-amyliod peptide, the major component of senile plaques in Alzheimer disease (AD), causes neuronal death through induction of oxidative stress. The medicinal plants from the Lamiaceae family contain the antioxidant activity. Moreover, neuroprotective properties of other species of this family have been reported. Materials and Methods: Aerial parts of the plants were extracted with ethanol and water using maceration method. Antioxidant activity of the extract was evaluated by 2, 2-diphenyl-1-picrylhydrazyl radical scavenging. PC12 cells were incubated with different concentrations of the extract in culture medium 1h prior to incubation with amyloid beta peptide. Cell toxicity and biomarkers of oxidative stress were assessed 24h after addition of amyloid beta peptide by MTT assay. Results: Butylated hydroxytoluene )BHT( at concentrations of 0.01, 0.1, and 1 µg/ml had higher anti-radical activity than Dracocephalum moldavica extract. However, at higher concentrations, plant extract showed a higher anti-radical activity than BHT. The plant extract at the concentration range of 0.01-100 µg/ml did not show any toxic effect on PC12 cells. The plant extract at concentrations of 1, 10, and 100 µg/ml protected cells against beta amyloid toxicity and at concentration of 10 µg/ml was able to prevent biomarkers changes of oxidative stress induced by beta amyloid. Conclusion: The extract of Dracocephalum moldavica prevents the toxicity of amyloid beta peptide by inhibiting the oxidative stress pathways and may useful in the treatment of AD.
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