The Neuroscience Journal of Shefaye Khatam
مجله علوم اعصاب شفای خاتم
Shefaye Khatam
Medical Sciences
http://shefayekhatam.ir
1
admin
2322-1887
2345-4814
10.61186/shefa
fa
jalali
1396
1
1
gregorian
2017
4
1
5
2
online
1
fulltext
en
P 32: The Role of CB2 Activation in Rats Under Harmaline Toxicity
P 32: The Role of CB2 Activation in Rats Under Harmaline Toxicity
تحقیقات پایه در علوم اعصاب
Basic research in Neuroscience
پژوهشي
Research --- Open Access, CC-BY-NC
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<p style="font-size: 12px; margin-top: 0px; margin-bottom: 0px; text-align: center;"><span style="color: rgb(0, 0, 0); font-size: 12px; line-height: 20.8px;">لطفاً به چکیده انگلیسی مراجعه شود.</span></p>
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<p><strong>Introduction:</strong> <em>β</em>-carbolines are shown to have significant anti-inflammatory effect via the inhibition of some inflammatory mediators including TNF-α and PGE2. In previous studies Purkinje cell deterioration have been proposed the dominant pathogenesis of harmaline toxicity. WIN55, 212-2 is a non-selective cannabinoid CB<sub>1</sub> and CB<sub>2</sub> receptor agonist. Combination of WIN55, 212-2 and AM 251(<a href="https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=2&cad=rja&uact=8&ved=0ahUKEwiI9Mjd3L7SAhXHOBQKHQMvDgEQFggbMAE&url=https%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpmc%2Farticles%2FPMC4234427%2F&usg=AFQjCNEn-48oVBAQ3grSiBP-POS2febBFA&sig2=LZGOocWiPOFBFX78CwvPGw">CB1-Selective Cannabinoid Receptor blocker</a>) can give us activation of CB2 receptor. In this study we aim to evaluate the possible protective effect of this combination against harmaline toxicity. <strong>Materials and Methods: </strong>30 rats (4 weeks aged) were kept in separate cages. They were randomly distributed into 3 groups. 1) Control, 2) harmaline (30 mg/kg according to our previous pilot study) and 3) WIN55, 212-2 (1mg/kg) +AM 251(1mg/kg) as cannabinoid receptor modulation. Agents were injected i.p. Open field test was used for evaluation of rat’s behavior including: mobility, Total distance movement (TDM), velocity, rearing and grooming. Also data collected from rotarod test to evaluate balance motor and balance performances and wire grip test (hanging) to asses muscle strength and balance. <strong>Results</strong>: Harmaline in this dose reliably affect a significant alteration in all body parts as severe tremor in which in the open field test severely decreases all parameters mentioned. On the other hand treatment with WIN55, 212-2 +AM251 increase both mobility (p<0.0027) and total distance movement (p< 0.0001) in contrast to harmaline. No significant differences were found in the velocity and rearing of harmaline group and WIN55, 212-2 +AM251. Also despite severe alteration in muscle strength and balance, there is no significant decrease in rod and hanging in rats treated by WIN1+AM251 compared to harmaline group. <strong>Conclusion:</strong> These results allow us to propose that treatment by WIN55,212-2 +AM251 in rats under harmaline toxicity have some positive effects on some aspects of movement by activation CB2 receptors but it needs further investigation on selective modulators and supplementary tests.</p>
Hrmalin, CB2, WIN55,212-2, AM251
63
63
http://shefayekhatam.ir/browse.php?a_code=A-10-24-881&slc_lang=en&sid=1
Nona
Sabeti
Nona
Sabeti
100319475328460011906
100319475328460011906
No
Student Research Committee, Faculty of Medicine, Mashhad University of Medical Science, Mashhad, Iran
Student Research Committee, Faculty of Medicine, Mashhad University of Medical Science, Mashhad, Iran
Hasan
Abbassian
Hasan
Abbassian
habasian@ymail.com
100319475328460011907
100319475328460011907
Yes
Department of Neuroscience, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Department of Neuroscience, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Benjamin
Jason Walley
Benjamin
Jason Walley
100319475328460011908
100319475328460011908
No
Department of Pharmacy, School of Chemistry, Food and Nutritional Sciences and Pharmacy, University of Reading, Whiteknights, Reading, Berkshire, RG6 6AP, UK
Department of Pharmacy, School of Chemistry, Food and Nutritional Sciences and Pharmacy, University of Reading, Whiteknights, Reading, Berkshire, RG6 6AP, UK
Mohammad
Shabani
Mohammad
Shabani
100319475328460011909
100319475328460011909
No
Kerman Neuroscience Research Center, Neuropharmacology Institute, Kerman University of Medical Sciences, Kerman, Iran
Kerman Neuroscience Research Center, Neuropharmacology Institute, Kerman University of Medical Sciences, Kerman, Iran