en O5: A Self-Assembled Nanopeptide Scaffold Combined with Mesenchymal Stem Cells Improved Functional Recovery after Traumatic Brain Injury in Rats تحقیقات پایه در علوم اعصاب Basic research in Neuroscience پژوهشي Research --- Open Access, CC-BY-NC <div style="text-align: center;"><span style="text-align: center; color: rgb(0, 0, 0); text-transform: none; text-indent: 0px; letter-spacing: normal; font-family: Tahoma; font-size: 12px; font-style: normal; font-weight: 400; word-spacing: 0px; float: none; display: inline !important; white-space: normal; orphans: 2; widows: 2; background-color: rgb(251, 250, 249); font-variant-ligatures: normal; font-variant-caps: normal; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial;">لطفاً به چکیده انگلیسی مراجعه شود.</span></div> <div style="text-align: justify;">Traumatic Brain Injury (TBI) is a major cause of death and disability worldwide. TBI can cause cognitive and memory function impairments which Current medical setting is not able to cure. In this study, we hypothesized that&nbsp;&nbsp; mesenchymal stem cells derived from Adipose tissue&nbsp; transplanted with RADA1- GGS IKVAV (GSIKVAV) can rescue cognitive function. An acute model of TBI was carried out in male Wistar rats (n=36). Subjects were divided into 7 groups: Sham (receiving no treatment), PBS, GSIKVAV, Mesenchymal Stem Cells (MSCs), GSIKVAV+ MSCs. MSCs were stained with BrdU. To evaluate the MSCs characterization flow cytometery was performed. IHC was done to study the differentiation and viability. Behavioral tests including Open Field (OF), Elevated Plus Maze (EPM) and modified Neurological Severity Score (mNSS) were done to evaluate the sensory- motor function and anxiety. At day 30, animals were sacrificed. In order to detect inflammation toll like receptor3, 4, tumor necrosis factor &alpha; and glial fibrillary acidic protein were assessed using western blot. CD markers (CD 105, 34) were reported to be positive for most of MSCs. The mNSS score significantly decreased among MSCs and MSCs + GSIKVAV groups. We observed a significant increase in the number of enteries to the open arm in the EPM test. The total distance was also increased in the OF test. These data suggest that using MSCs with GSIKVAV can be a potential therapy for TBI patients according its neuroprotective and immunomodulatory effects.<br> &nbsp;</div> Tissue Engineering, Neuroinflammation, Functional Recovery 5 5 http://shefayekhatam.ir/browse.php?a_code=A-10-24-1063&slc_lang=en&sid=1 Farzin Naseri Farzin Naseri 100319475328460015353 100319475328460015353 No Department of Neuroscience, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Department of Neuroscience, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Mohammad Moein Shirzad Mohammad Moein Shirzad 100319475328460015354 100319475328460015354 No Department of Neuroscience, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Department of Neuroscience, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Ghazale Biglari Ghazale Biglari 100319475328460015355 100319475328460015355 No Department of Neuroscience, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Department of Neuroscience, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Ali Hasani Dooghabadi Ali Hasani Dooghabadi 100319475328460015356 100319475328460015356 No Department of Neuroscience, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Department of Neuroscience, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Ali Jahanbazi Jahan Abad Ali Jahanbazi Jahan Abad 100319475328460015357 100319475328460015357 No Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran Arezou Es'haghabadi Arezou Es'haghabadi 100319475328460015358 100319475328460015358 No Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran Ali Gorji Ali Gorji 100319475328460015359 100319475328460015359 No a. Department of Neurosurgery, Westfälische Wilhelms-Universität Münster, Münster, Germany b. Epilepsy Research Center, Westfälische Wilhelms-Universität Münster, Robert-Koch-Straße 45, 48149 Münster, Germany a. Department of Neurosurgery, Westfälische Wilhelms-Universität Münster, Münster, Germany b. Epilepsy Research Center, Westfälische Wilhelms-Universität Münster, Robert-Koch-Straße 45, 48149 Münster, Germany Sajad Sahab Negah Sajad Sahab Negah sahabnegahs@mums.ac.ir 100319475328460015360 100319475328460015360 Yes Department of Neuroscience, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Department of Neuroscience, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran