en O15: Using Stromal Cell-Derived Factor-I as Bio Active Motif in A Novel Self-Assembly Peptide Nanofiber Scaffold: an Approach to Improve Cell Therapy in Brain Injury تحقیقات پایه در علوم اعصاب Basic research in Neuroscience پژوهشي Research --- Open Access, CC-BY-NC <div style="text-align: center;"><span style="text-align: center; color: rgb(0, 0, 0); text-transform: none; text-indent: 0px; letter-spacing: normal; font-family: Tahoma; font-size: 12px; font-style: normal; font-weight: 400; word-spacing: 0px; float: none; display: inline !important; white-space: normal; orphans: 2; widows: 2; background-color: rgb(251, 250, 249); font-variant-ligatures: normal; font-variant-caps: normal; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial;">لطفاً به چکیده انگلیسی مراجعه شود.</span></div> <p style="text-align: justify;">Traumatic brain injury (TBI) is one of the main causes of mortality and morbidity worldwide. Despite extensive investigations over the past few decades, no effective therapies exist to improve the brain function in patients with TBI. Neural tissue engineering is an attractive therapeutic approach to restore the brain structure and function of damaged tissue. Bioactive motif of Stromal cell-derived factor-1 (SDF-1) induces neurogenesis by increasing the migration and proliferation of endogenous neural progenitor cells (NPCs) in the lesion sites. We designed the novel scaffold with SDF1 and RADA16. The Aim of this study is to determine in vitro effects of SDF scaffold on neural stem cells behavior including migration, attachment, and differentiation. Neural stem cells were isolated from the hippocampus and subventricular zone of the lateral ventricle of 17-days rat fetus. In this study, Apoptosis, cytotoxicity, proliferation, neurite outgrowth, and differentiation were assessed. Migration, attachment and differentiation of stem cells significantly increased in the SDF scaffold. Our results showed no significant difference between apoptosis, survival and proliferation of cells in SDF and RADA16-IKVAV scaffolds. Although SDF scaffold increased Migration, and attachment of stem cells in vitro, in vivo studies should be conducted to determine the features of SDF scaffold in the brain tissue.</p> Neural Tissue Engineering, Stromal Derived Factor, Neural Stem Cells 15 15 http://shefayekhatam.ir/browse.php?a_code=A-10-24-1081&slc_lang=en&sid=1 Mohsen Saberifar Mohsen Saberifar 100319475328460015396 100319475328460015396 No Neuroscience Department, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Neuroscience Department, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Mohammad Amin Bayat Tork Mohammad Amin Bayat Tork 100319475328460015397 100319475328460015397 No Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran Ali Gorji Ali Gorji 100319475328460015398 100319475328460015398 No a. Neuroscience Department, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran b. Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran c. Department of Neurosurgery, Westfälische Wilhelms-Universität Münster, Münster, Germany d. Epilepsy Research Center, Westfälische Wilhelms-Universität Münster, Robert-Koch-Straße 45, 48149 Münster, Germany a. Neuroscience Department, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran b. Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran c. Department of Neurosurgery, Westfälische Wilhelms-Universität Münster, Münster, Germany d. Epilepsy Research Center, Westfälische Wilhelms-Universität Münster, Robert-Koch-Straße 45, 48149 Münster, Germany Sajad Sahab Negah Sajad Sahab Negah sahabnegahs@mums.ac.ir 100319475328460015399 100319475328460015399 Yes a. Neuroscience Department, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran b. Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran a. Neuroscience Department, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran b. Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran