en O27: Interaction of Cancer Stem Cells and Microglia in Glioblastoma Multiforme تحقیقات پایه در علوم اعصاب Basic research in Neuroscience پژوهشي Research --- Open Access, CC-BY-NC <div style="text-align: center;"><span style="text-align: center; color: rgb(0, 0, 0); text-transform: none; text-indent: 0px; letter-spacing: normal; font-family: Tahoma; font-size: 12px; font-style: normal; font-weight: 400; word-spacing: 0px; float: none; display: inline !important; white-space: normal; orphans: 2; widows: 2; background-color: rgb(251, 250, 249); font-variant-ligatures: normal; font-variant-caps: normal; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial;">لطفاً به چکیده انگلیسی مراجعه شود.</span></div> <div style="text-align: justify;">Malignant gliomas are highly invasive brain tumors with the occurrence of multiple microglia/macrophages in the tumor microenvironment. Macrophages/microglia that found in glioma microenvironment, as tumor-infiltrating immune cells, can play a harmful role in tumor progression. In addition, glioblastoma multiforme (GBM) contains multiple aberrant differentiation and tumorigenic cancer stem cells (CSCs) that contribute to tumor heterogeneity and resistance to anti-cancer therapies. The present study was aimed to understand the interaction between microglial cells and CSCs in a co-culture system. Specific markers used for the characterization of CSCs and microglia in GBM tissues obtained from patients. Then, we applied a co-culture system consisting of permeable membrane allowing secreted soluble factors to diffuse. Measuring the effects of cytokines secreted by activated and non-activated microglia on CSCs, MTS cell proliferation assay were performed. Cell viability in CSCs treated with non-activated microglia was significantly reduced compared to the group that treated with activated microglia. The activated microglia/macrophages may interfere in the process of tumor angiogenesis, metastasis niches, recurrence and support tumour invasiveness.<br> &nbsp;</div> Microglia, Cancer Stem Cells, Cell Culture 27 27 http://shefayekhatam.ir/browse.php?a_code=A-10-24-1101&slc_lang=en&sid=1 Sara Abdolahi Sara Abdolahi 100319475328460015427 100319475328460015427 No Shefa Neuroscience Research Center, Khatam-Alanbia Hospital, Tehran, Iran Shefa Neuroscience Research Center, Khatam-Alanbia Hospital, Tehran, Iran Sepideh Ghasemi Sepideh Ghasemi 100319475328460015428 100319475328460015428 No Shefa Neuroscience Research Center, Khatam-Alanbia Hospital, Tehran, Iran Shefa Neuroscience Research Center, Khatam-Alanbia Hospital, Tehran, Iran Amir Azarhomayoun Amir Azarhomayoun 100319475328460015429 100319475328460015429 No Sina Trauma and Surgery Research Center, Tehran University of Medical Sciences, Tehran, Iran Sina Trauma and Surgery Research Center, Tehran University of Medical Sciences, Tehran, Iran Ali Gorji Ali Gorji gorjial@uni-muenster.de 100319475328460015430 100319475328460015430 Yes a. Shefa Neuroscience Research Center, Khatam-Alanbia Hospital, Tehran, Iran b. Department of Neurosurgery, Westfälische Wilhelms-Universität Münster, Münster, Germany c. Department of Neuroscience, Mashhad University of Medical Sciences, Mashhad, Iran a. Shefa Neuroscience Research Center, Khatam-Alanbia Hospital, Tehran, Iran b. Department of Neurosurgery, Westfälische Wilhelms-Universität Münster, Münster, Germany c. Department of Neuroscience, Mashhad University of Medical Sciences, Mashhad, Iran