en P34: Berberin Exerts Neuroprotective Effects by Modulating Pro and Anti-Inflammatory Cytokines in Rat Model of MCAO تحقیقات پایه در علوم اعصاب Basic research in Neuroscience پژوهشي Research --- Open Access, CC-BY-NC &nbsp; <table border="0" cellpadding="5" cellspacing="2" dir="rtl" id="table_article" style="text-transform: none; text-indent: 0px; letter-spacing: normal; font-family: Tahoma; word-spacing: 0px; orphans: 2; widows: 2; break-inside: avoid !important; background-color: rgb(251, 250, 249); -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial;" width="100%"> <tbody style="text-align: justify; line-height: 2.2; font-size: 12px; margin-top: 0px; margin-bottom: 0px; break-inside: avoid !important;"> <tr style="break-inside: avoid !important;"> <td class="abstractmed" colspan="2" style="text-align: justify; break-inside: avoid !important;"> <p style="text-align: center; font-size: 12px; margin-top: 0px; margin-bottom: 0px;"><span style="text-align: center; color: rgb(0, 0, 0); font-family: Tahoma; font-size: 12px; background-color: rgb(251, 250, 249);">لطفاً به چکیده انگلیسی مراجعه شود.</span></p> </td> </tr> <tr style="break-inside: avoid !important;"> </tr> </tbody> </table> <p style="text-align: justify;">Many complicated mechanisms are involved in brain ischemia and the role of inflammatory factors in the progression of post-ischemic injury is inevitable. In present study, anti-inflammatory effect of berberine has been investigated in reperfusion injury after acute ischemic stroke. Male Wistar rats weighing 250-270 gr were randomly divided into four cohorts: healthy rats (control, n=20), sham-operated animals (sham, n=20), ischemia/reperfusion (I/R) cohort (MCAO=20), I/R+ berberine cohort (MCAO+ berberine, n=20). The animal subjected to ischemia for 45 min and berberine (40mg/kg) was interaperitoneally administrated 1 h after reperfusion. At 24 h after reperfusion, the animals were sacrificed under deep anesthesia and expression changes of pro and anti-inflammatory cytokines were determined by immunohistochemical assay. To evaluate brain edema, brain water content was measured in experimental groups 24 h after reperfusion. The infarct size was determined using 3, 5-Triphenyltetrazolium chloride (TTC) staining. significant increased levels of pro-inflammatory cytokines (IL1&beta; and TNF&alpha;) were found in MCAO cohort compared with control and sham cohorts. Likewise, expression level of anti-inflammatory cytokines (IL10) was slightly increased following I/R in MCAO cohort. berberine conferred a neuro-protective effect via reduction of infarct size, preventing brain edema and significant down-regulation of pro-inflammatory cytokines and up-regulation of anti-inflammatory cytokines. In sum, our findings demonstrate that berberine post-treatment attenuates focal cerebral IR injury by targeting inflammation process. This opens up potential avenues for future research and treatment of ischemic stroke.</p> Berberine, Inflammatory Cytokines, MCAO Model, Neuroprotection 65 65 http://shefayekhatam.ir/browse.php?a_code=A-10-24-1172&slc_lang=en&sid=1 Solmaz Nasseri Maleki Solmaz Nasseri Maleki snms1369@gmail.com 100319475328460016117 100319475328460016117 Yes Physiology Research Center, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran Physiology Research Center, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran Nahid Aboutaleb Nahid Aboutaleb 100319475328460016118 100319475328460016118 No Physiology Research Center, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran Physiology Research Center, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran