The Neuroscience Journal of Shefaye Khatam
مجله علوم اعصاب شفای خاتم
Shefaye Khatam
Medical Sciences
http://shefayekhatam.ir
1
admin
2322-1887
2345-4814
10.61186/shefa
fa
jalali
1397
1
1
gregorian
2018
4
1
6
2
online
1
fulltext
en
P175: Neonatal Homocysteine Administration Induces Cerebellar Toxicity Via Oxidative Stress in Rats
P175: Neonatal Homocysteine Administration Induces Cerebellar Toxicity Via Oxidative Stress in Rats
تحقیقات پایه در علوم اعصاب
Basic research in Neuroscience
پژوهشي
Research --- Open Access, CC-BY-NC
<div style="text-align: center;"><span style="background:#FBFAF9;"><span style="color:black;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:9.0pt;">لطفاً به چکیده انگلیسی مراجعه شود</span></span></span></span><span dir="LTR" style="background:#FBFAF9;"><span style="color:black;"><span style="font-family:tahoma,sans-serif;"><span style="font-size:9.0pt;">.</span></span></span></span></div>
<div style="text-align: justify;">Several in vitro studies have showed the neurotoxic effect of homocysteine (Hcy) to various neuronal types, including cerebellar Purkinje neurons. In this study, we investigated whether the Hcy has neurotoxic effects on the rat pup’s cerebellum in the postnatal period that it continues to development. Pups rats divided into control and Hcy group. Hcy administration (0.3–0.6 mmol/g body weight) was initiated on postnatal day (PD) 4 and continued until PD 25 by subcutaneous injection twice on a day with 8 hours interval. On PD 25, the animals were decapitated and the cerebellums were removed. The body and cerebellum weight were measured and the cerebellum to body weight ratio was calculated by dividing the cerebellum weight by the body weight of pups at sacrifice. Level of lipid peroxidation, the glutathione peroxidase (GPx) activity in the cerebellum were determined. Also, caspase3 protein level was assayed by Elisa kit in the cerebellum. Our results demonstrated that chronic administration Hcy significantly decreased GPx activity and increased lipid peroxidation in the cerebellum. The cerebellum weight and the cerebellum to body weight ratio significantly reduced in Hcy treated rat compared with the control group. Moreover, Hcy increased caspase 3 protein levels in Hcy treated group. Our results showed systemic administration of Hcy induces cerebellar toxicity through oxidative stress.</div>
Homocystein, Oxidative Stress, Cerebellum
206
206
http://shefayekhatam.ir/browse.php?a_code=A-10-24-1251&slc_lang=en&sid=1
Hakimeh
Koohpeyma
Hakimeh
Koohpeyma
h.kohpeyma20@gmail.com
100319475328460016289
100319475328460016289
Yes
Department of Physiology, Faculty of Biology, Damghan University, Damghan, Iran
Department of Physiology, Faculty of Biology, Damghan University, Damghan, Iran
Iran
Goudarzi
Iran
Goudarzi
100319475328460016290
100319475328460016290
No
Department of Physiology, Faculty of Biology, Damghan University, Damghan, Iran
Department of Physiology, Faculty of Biology, Damghan University, Damghan, Iran
Mahmoud
Elahdadi Salman
Mahmoud
Elahdadi Salman
100319475328460016291
100319475328460016291
No
Department of Physiology, Faculty of Biology, Damghan University, Damghan, Iran
Department of Physiology, Faculty of Biology, Damghan University, Damghan, Iran
Taghi
Lashkarblouki
Taghi
Lashkarblouki
100319475328460016292
100319475328460016292
No
Department of Biochemistry, Faculty of Biology, Damghan University, Damghan, Iran
Department of Biochemistry, Faculty of Biology, Damghan University, Damghan, Iran