The Neuroscience Journal of Shefaye Khatam
مجله علوم اعصاب شفای خاتم
Shefaye Khatam
Medical Sciences
http://shefayekhatam.ir
1
admin
2322-1887
2345-4814
10.61186/shefa
fa
jalali
1393
9
1
gregorian
2014
12
1
2
4
online
1
fulltext
fa
O23: Polytrauma with and without Neurotrauma: Experimental Animal Studies with Clinical, Immunological and Histopathological Features
O23: Polytrauma with and without Neurotrauma: Experimental Animal Studies with Clinical, Immunological and Histopathological Features
نوروپاتولوژي
Neuropathology
پژوهشي
Research --- Open Access, CC-BY-NC
<p align="center">لطفاً به چکیده انگلیسی مراجعه شود. </p>
Although the majority of the patients with traumatic brain injury presents with injuries involving the extremities, there was a clear paucity of adequate experimental polytrauma models. To close this gap, we conducted several studies to establish two different polytrauma mice models including both closed and open traumatic brain injury. Male C57BL mices with a mean weight of 23g were anesthetized with ketamine and xylazine. The anaesthetized animals were subjected to a controlled cortical impact (CCI) (n=20) over the left parietotemporal cortex using rounded-tip impounder for application of a standardized brain injury. Following fracture of the right femur using a guillotine, a hemorrhagic shock was induced via blood aspiration. The control groups included animals with isolated CCI (n=20) and animals with fracture of femur plus hemorrhagic shock (n=20). The second model included weight-drop injury with femure fracture and hemorrhagic shock (n=20), isolated weight-drop (n=20), femure fracture and hemorrhagic shock (n=20). All subjects were sacrified 96 hours following trauma. Intracardial blood samples were taken before. Brain, kidney, liver, lung and spleen were taken for histopathological examination.
The inflammatory response measured by Il-6, TNFa, CD4+ and CD+8 cells was stronger in the polytrauma group, in comparison with the control groups. Within the histopathological investigations, polytraumatized animals differ from those with a single trauma (traumatic brain injury or femur fracture with hemorrhagic shock) with various severity. The findings of the studies show that such polytrauma models can be standardized resulting in a reproducible damage. These models fulfill the requirements of a standardized animal model. It allows adequate analogies and inferences to the clinical situation of polytrauma in humans. It opens promising capabilities for more evaluation of possible posttraumatic drug therapy, hetereotopic ossification and also studies with knock-out mice.
Polytrauma Models, Immunological, Histopathological
23
23
http://shefayekhatam.ir/browse.php?a_code=A-10-24-368&slc_lang=fa&sid=1
M. Javad
Mirzayan
M. Javad
Mirzayan
mirzayan@hotmail.com
10031947532846005705
10031947532846005705
Yes
Department of Neurosurgery, Medical School Hannover, Hannover, Germany.
Department of Neurosurgery, Medical School Hannover, Hannover, Germany.
Christian
Probst
Christian
Probst
10031947532846005706
10031947532846005706
No
Department of Traumatology, Medical School Hannover, Hannover, Germany.
Department of Traumatology, Medical School Hannover, Hannover, Germany.