The Neuroscience Journal of Shefaye Khatam
مجله علوم اعصاب شفای خاتم
Shefaye Khatam
Medical Sciences
http://shefayekhatam.ir
1
admin
2322-1887
2345-4814
10.61186/shefa
fa
jalali
1393
9
1
gregorian
2014
12
1
2
4
online
1
fulltext
fa
P39: Effect of Injured Brain Extract on Proliferation of Neural Stem/Progenitor Cells Cultured in a 3-dimensional Scaffold
P39: Effect of Injured Brain Extract on Proliferation of Neural Stem/Progenitor Cells Cultured in a 3-dimensional Scaffold
تحقیقات پایه در علوم اعصاب
Basic research in Neuroscience
پژوهشي
Research --- Open Access, CC-BY-NC
<p align="center">لطفاً به چکیده انگلیسی مراجعه شود. </p>
Neuroinflamation occurring after traumatic brain injury (TBI) is a complex phenomenon comprising distinct cellular and molecular events involving the injured cerebral tissue. Mechanical injury to adult rat brain elucidated the accumulation in the affected area of trophic activity. In the tissue adjacent to the injury, trophic titers began to rise after the lesion. This study aimed to determine the effect of injured brain extract on proliferation of neonatal rat neural stem/progenitor cells (NS/PCs). NS/PCs were isolated from olfactory bulb of neonatal rat then cultured as neurospheres. After second passage, cells were seeded in PuraMatrix scaffold by injection method and incubated with intact or injured brain extracts. The proliferation assay was carried out by MTS assay 10 days later. For preparation of the injured brain extract, we used a rat brain injury model and collected the extract 48 hrs after injury. The experimental results showed that NS/PCs derived olfactory bulb have a good potential to proliferate. MTS assay demonstrated that NP/SCs proliferation rate was increased in injured brain extract. Injured brain extract could be benefit for NP/SCs proliferation.
Brain Extract, Proliferation, Scaffold
89
89
http://shefayekhatam.ir/browse.php?a_code=A-10-24-435&slc_lang=fa&sid=1
Sajad
Sahab Negah
Sajad
Sahab Negah
10031947532846005818
10031947532846005818
No
a. Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran. b. Histology and Embryology Group, Basic Science Department, Veterinary Medicine Faculty, Shiraz University, Shiraz, Iran.
a. Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran. b. Histology and Embryology Group, Basic Science Department, Veterinary Medicine Faculty, Shiraz University, Shiraz, Iran.
Hadi
Aligholi
Hadi
Aligholi
hadialigholi@yahoo.com
10031947532846005819
10031947532846005819
Yes
Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran.
Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran.
Shahin
Mohammad Sadeghi
Shahin
Mohammad Sadeghi
10031947532846005820
10031947532846005820
No
Department of Plastic and Reconstructive Surgery, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Plastic and Reconstructive Surgery, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Mostafa
Modarres Mousavi
Mostafa
Modarres Mousavi
10031947532846005821
10031947532846005821
No
Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran.
Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran.