The Neuroscience Journal of Shefaye Khatam
مجله علوم اعصاب شفای خاتم
Shefaye Khatam
Medical Sciences
http://shefayekhatam.ir
1
admin
2322-1887
2345-4814
10.61186/shefa
fa
jalali
1393
9
1
gregorian
2014
12
1
2
4
online
1
fulltext
fa
P71: Toward Treatment of Post-Traumatic Spinal Cord Injury: Differentiation of Oligodendrocytes from Adipose Stem Cells
P71: Toward Treatment of Post-Traumatic Spinal Cord Injury: Differentiation of Oligodendrocytes from Adipose Stem Cells
تحقیقات پایه در علوم اعصاب
Basic research in Neuroscience
پژوهشي
Research --- Open Access, CC-BY-NC
<p align="center">لطفاً به چکیده انگلیسی مراجعه شود. </p>
Traumatic spinal cord injuries (SCIs) lead to severe and permanent neurological deficits. Although no effective therapeutic option is currently available, recent other studies have shown that cell transplantation strategies hold promise to enhance functional recovery after SCI. Adipose stem cells (ADSCs) obtained from pararenal and inguinal region of rats. ADSCs were cultured and then differentiated to the neural stem cells (NSCs). Directed differentiation of stem/progenitor cells to oligodendrocytes (OLCs) lineage was done and the cell viability was assessed by trypan blue. Immunocytochemistry was carried out by NF68, NF160, fibronectin and nestin for NSCs. Also, it was done by O4, O1, and oligo2 for OLCs. Results showed that fibronectin, CD44, CD90 and CD45 expressed 94.32±0.45%, 95.48±0.24% and 97.16±0.82% respectively. Expression of O1, O4 and oligo2 showed that combination of HRG, PDGF, bFGF and T3 (25 ng/ml) have an effective role in transdifferentiation of ADSCs into OLCs. ADSCs can differentiate to mature OLCs. Our suggestion is that oligodendrocytes can be used as a therapeutic strategy for treatment of SCI in future.
Oligodendrocytes, Neurological Disorders, Fibronectin
121
121
http://shefayekhatam.ir/browse.php?a_code=A-10-24-469&slc_lang=fa&sid=1
Azadeh
Sajadian
Azadeh
Sajadian
10031947532846005953
10031947532846005953
No
Shefa Neuroscience Research Center, Khatam Alanbia Hospital,Tehran, Iran.
Shefa Neuroscience Research Center, Khatam Alanbia Hospital,Tehran, Iran.
Leila
Alizadeh
Leila
Alizadeh
l.alizadehh@yahoo.com
10031947532846005954
10031947532846005954
Yes
Shefa Neuroscience Research Center, Khatam Alanbia Hospital,Tehran, Iran.
Shefa Neuroscience Research Center, Khatam Alanbia Hospital,Tehran, Iran.