Introduction: Several vital body systems, such as visual and auditory systems, can be influenced by fatigue. Each system can be affected based on the complexity of its tasks and the level of involvement in information processing in the brain. The aim of this study was to evaluate the effect of fatigue on visual and auditory reaction times after an exhaustive exercise. Materials and Methods: 15 female students of Shahid Rajaee University participated in this study. Bruce test was used to make general fatigue. The dependent variables include visual and auditory reaction times that compared in fatigue and non-fatigue conditions. Kolmogorov- Smirnov test was used for considering data distribution and Paired t test was used for considering the differences between groups. Results: The results revealed a significant increase in visual and auditory reaction times in fatigue conditions. However, the data showed that fatigue has the same effect on the visual and auditory reaction times. Conclusion: The results showed that general fatigue influenced equally the visual and auditory systems.