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Shefaye Khatam 2018, 6 - The Spring Supplement of Shefaye Khatam 1 -: 61-61 Back to browse issues page
P30: Effects of Hemin on Ca2+Influx in Neurons of C57BL/6 Mouse Brain
Hamid Islampoor *
Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran , bidgani777@yahoo.com
Abstract:   (829 Views)
Excitotoxicity results in a significant increase in Ca2+ influx; essentially from open N-Methyl-D-aspartate receptors (NMDARs) channels that cause a secondary rise in the intracellular Ca2+ concentration. It is correlated with neuronal death induced by Ca2+ overload. Dysfunction of NMDARs is associated with excitotoxic neuronal death in neurodegenerative disorders. In this study, the effects of hemin on Ca2+ permeability in neurons of C57BL/6 mouse brain examined. Isolated from 1-dayold C57BL/6 mice, were cultured in serum-free media. Cells were maintained in growth medium at 37◦C in 95% air/5% CO2 for 2 weeks in vitro before treatment. Primary neurons were cultured in serum-free media were treated with hemin (0, 12.5, 25, 50, 75,100 μM) for 18 (h). Intensity of calcium fluorescence was reduced in treated cultures with hemin (100, 86, 78.5, 60, 56, 46%, respective to the concentrations stated previously; P<0.05 for all). Hemin increased Ca2+ influx in cultured neurons. NMDAR stimulation by hemin increased the activating of NMDARs and Ca2+ influx in the cultured neurons. Therefore, heminis cytotoxic due to increase of intracellular Ca2+ influx.
 
Keywords: Mouse, Hemin, Neurons, Treatment
Full-Text [PDF 239 kb]   (206 Downloads)    
Type of Study: Research --- Open Access, CC-BY-NC | Subject: Basic research in Neuroscience


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Islampoor H. P30: Effects of Hemin on Ca2+Influx in Neurons of C57BL/6 Mouse Brain. Shefaye Khatam. 2018; 6 (S1) :61-61
URL: http://shefayekhatam.ir/article-1-1621-en.html


Volume 6 - The Spring Supplement of Shefaye Khatam 1 - Back to browse issues page
مجله علوم اعصاب شفای خاتم The Neuroscience Journal of Shefaye Khatam
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