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:: Volume 9, Issue 3 (Summer 2021) ::
Shefaye Khatam 2021, 9(3): 55-63 Back to browse issues page
Synthesis, Characterization, Evaluation of Supportive Properties, and Neuroprotective Effects of Cerium Oxide Nanoparticles as a Candidate for Neural Tissue Engineering
Yasaman Arzanipur, Arash Abdolmaleki *, Asadollah Asadi, Saber Zahri
a. Department of Engineering Sciences, Faculty of Advanced Technologies, University of Mohaghegh Ardabili, Namin, Iran. b. Bio Science and Biotechnology Research center (BBRC), Sabalan University of Advanced Technologies (SUAT), Namin, Iran , abdolmalekiarash1364@gmail.com
Abstract:   (887 Views)
Introduction: Tissue engineering is a part of biotechnology that includes the development of biological implants for tissue regeneration to improve tissue or organ function. This study aimed to investigate the effect of cerium oxide nanoparticles on the interactions between adipose tissue stem cells and decellularized sciatic nerve scaffolds in rats. Materials and Methods: Rats were anesthetized by injecting a mixture of ketamine (80 mg/kg) and xylazine (10 mg/kg). Sciatic nerve fragments (15 mm) were removed above the three-pronged site in the thigh muscle and decellularized after cleaning the surrounding tissues using the Sandal method. Then, adipose tissue mesenchymal cells were implanted on the scaffold, and the growth and viability of the cells implanted on the scaffold in the presence of cerium oxide nanoparticles were measured by MTT assay. Results: The results of histological evaluations showed that the scaffolds were completely decellularized and hematoxylin/eosin and Dapi staining confirmed these results. Specialized tissue evaluation by Masson trichrome staining as well as biomechanical analysis showed that collagen and elastin fibers were relatively preserved in the extracellular matrix. Cell viability on the scaffold increased in the presence of nanoparticles. Conclusion: Cerium oxide nanoparticles increase cell stability, proliferation, and maintenance of adipose tissue mesenchymal cells and may be beneficial in the treatment of peripheral nerve lesions.
Keywords: Sciatic Nerve, Cerium, Extracellular Matrix, Regeneration, Tissue Engineering
Full-Text [PDF 958 kb]   (195 Downloads)    
Type of Study: Research --- Open Access, CC-BY-NC | Subject: Neural Repair
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Arzanipur Y, Abdolmaleki A, Asadi A, Zahri S. Synthesis, Characterization, Evaluation of Supportive Properties, and Neuroprotective Effects of Cerium Oxide Nanoparticles as a Candidate for Neural Tissue Engineering. Shefaye Khatam. 2021; 9 (3) :55-63
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Volume 9, Issue 3 (Summer 2021) Back to browse issues page
مجله علوم اعصاب شفای خاتم The Neuroscience Journal of Shefaye Khatam
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