[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit ::
Main Menu
Home::
Journal Information::
Articles Archive::
Guide for Authors::
For Reviewers::
Ethical Statements::
Registration::
Site Facilities::
Contact us::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
Copyright Policies

 

AWT IMAGE

 

..
Open Access Policy

This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.

..
:: Volume 11, Issue 2 (Spring 2023) ::
Shefaye Khatam 2023, 11(2): 75-80 Back to browse issues page
The Split Well: a New Method for Comparing Nano-Scaffolds in Electron Microscopy
Javad Momeni , Mehdi Hadi Jafari , Hadi Aligholi , Sajad Sahab Negah *
a. Neuroscience Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. b. Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran , Sahabnegahs@mums.ac.ir
Abstract:   (837 Views)
Introduction: Comparing the nanostructure of multiple scaffolds for tissue engineering using electron microscopy poses significant challenges in terms of time and cost. To overcome this, we have developed a novel method that enables the assessment of two different scaffolds on a single slide in scanning electron microscopy (SEM). Materials and Methods: The Pura Matrix scaffold was used to study this method. Firstly, each scaffold was positioned at the bottom of a well, ensuring that every sample was placed on one specific side of the well. Then, each sample was washed with PBS and fixed with glutaraldehyde. After the scaffolds were fixed, they were washed three times with PBS and finally dried overnight at room temperature. After 24 hours, the samples were coated in gold and then observed using SEM. Results: In this study, we utilized this method successfully to investigate the structures of different self-assembling peptides. Our split-well approach not only saves time for researchers and reduces confounding variables but also minimizes the cost of services by allowing simultaneous evaluation of two scaffolds. Conclusion: Our findings demonstrate that researchers can achieve precise comparisons using similar magnifications. Overall, the split-well approach proves to be a cost-effective and accurate method for comparing different scaffolds in tissue engineering.
Keywords: Cell Culture Techniques, Tissue Engineering, Methods
Full-Text [PDF 1389 kb]   (634 Downloads)    
Type of Study: Research --- Open Access, CC-BY-NC | Subject: Basic research in Neuroscience
References
1. Inkson BJ. 2 - Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) for materials characterization. In: Hübschen G, Altpeter I, Tschuncky R, Herrmann H-G, editors. Materials Characterization Using Nondestructive Evaluation (NDE) Methods: Woodhead Publishing; 2016. p. 17-43. [DOI:10.1016/B978-0-08-100040-3.00002-X]
2. Lewczuk B, Szyryńska N. Field-Emission Scanning Electron Microscope as a Tool for Large-Area and Large-Volume Ultrastructural Studies. Animals : an open access journal from MDPI. 2021;11(12). [DOI:10.3390/ani11123390]
3. Fischer ER, Hansen BT, Nair V, Hoyt FH, Dorward DW. Scanning electron microscopy. Current protocols in microbiology. 2012; Chapter 2: Unit 2B. [DOI:10.1002/9780471729259.mc02b02s25]
4. Meller K. General methods in scanning electron microscopy of the nervous system. Springer; 1981. [DOI:10.1007/978-3-642-68029-8_4]
5. Scanning Electron Microscope Internet2023 [Available from: https://www.microscopemaster.com/scanning-electron-microscope.html.
6. Balzano A, Merela M, Čufar K. Scanning electron microscopy protocol for studying anatomy of highly degraded waterlogged archaeological wood. Forests. 2022; 13(2): 161. [DOI:10.3390/f13020161]
7. Mehdizadeh KA, Tahermanesh K, Chaichian S, Joghataei MT, Moradi F, Tavangar SM, et al. How to prepare biological samples and live tissues for scanning electron microscopy (SEM). 2014. [DOI:10.31661/gmj.v3i2.267]
8. Mohammed A, Abdullah A, editors. Scanning electron microscopy (SEM): A review. Proceedings of the 2018 International Conference on Hydraulics and Pneumatics-HERVEX, Băile Govora, Romania; 2018.
9. Sahab Negah S, Khaksar Z, Aligholi H, Mohammad Sadeghi S, Modarres Mousavi SM, Kazemi H, et al. Enhancement of neural stem cell survival, proliferation, migration, and differentiation in a novel self-assembly peptide nanofibber scaffold. Molecular neurobiology. 2017; 54: 8050-62. [DOI:10.1007/s12035-016-0295-3]
10. University I. Electron Microscopy Pricing Internet2023 [Available from: https://medicine.iu.edu/service-cores/facilities/electron-microscopy/pricing.
11. Rayan P. SEM Lab Internet 2023 [Available from: https://partowrayan.com/nano-lab/sem-lab/.



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Momeni J, Hadi Jafari M, Aligholi H, Sahab Negah S. The Split Well: a New Method for Comparing Nano-Scaffolds in Electron Microscopy. Shefaye Khatam 2023; 11 (2) :75-80
URL: http://shefayekhatam.ir/article-1-2402-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 11, Issue 2 (Spring 2023) Back to browse issues page
مجله علوم اعصاب شفای خاتم The Neuroscience Journal of Shefaye Khatam
Persian site map - English site map - Created in 0.05 seconds with 45 queries by YEKTAWEB 4652