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Identifying Mechanisms of Radiation Resistance in Glioblastoma Using Bioinformatic and Molecular-Cellular Approaches
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Arezou Eshaghadadi   , Taereh Foroutan * , Ali Gorji , Noormohammad Meshkinkhood |
| Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran , foroutan@khu.ac.ir |
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Abstract: (38 Views) |
Introduction: Glioblastoma (GBM) represents the most common and aggressive malignant brain tumor in adults, characterized by poor responsiveness to both radiotherapy and chemotherapy. The average survival duration for affected patients ranges from 14 to 20 months. This study aims to elucidate the molecular underpinnings of radiation resistance in GBM by integrating bioinformatic analyses with experimental laboratory approaches. Materials and Methods: Analysis of gene expression datasets identified four candidate genes (KLF10, THBS1, NFYB, OSR1). Human GBM cells were cultured, irradiated with 6 Gy, and processed for RNA isolation and qPCR to evaluate gene expression. Results: Our results showed that OSR1 expression was significantly increased, while THBS1 levels were decreased in radiation-resistant cells compared to control samples; however, KLF10 and NFYB did not exhibit significant changes. Conclusion: These findings implicate OSR1 and THBS1 as potential biomarkers of radiation resistance. Further investigations into their pathways could help in developing targeted therapies for GBM.
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| Keywords: Glioma, Cell Culture Techniques, Computational Biology, Genes |
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Full-Text [PDF 2586 kb]
(18 Downloads)
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Type of Study: Research --- Open Access, CC-BY-NC |
Subject:
Molecular Neurobiology
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