Introduction: Schizophrenia begins typically in young adulthood (before 25 years old) and about 1% of people are affected during their lifetime. The antipsychotic drugs have several side effects. This study was aimed to investigate the Mozart’s music effect, as a non-invasive treatment, on the schizophrenia. Materials and Methods: Rats aged 21-25 days were divided into four groups: control, music-treated, social isolated (SI) and SI + music-treatment groups. Animals in the control group, without music exposure and music-treated rats, with music exposure, were housed socially. Animals in the SI group, without music exposure, and SI + music-treatment were housed individually for 6 weeks. Music (Mozart’s piano sonata, KV361) was played 24 hours before and during behavioral tests. Social contraction test for each rat was performed. The positive and negative behaviors were scored. Results: The mean number of negative behaviors, such as pursuit, attack, biting and offensive behaviors, in SI and SI + music-treatment increased compared to control and music-treated groups. In addition, biting, up right offensive and treating in SI + music-treatment significantly decreased compared to SI group. In addition, the positive behaviors (walking) in SI + music-treatment group significantly increased compared to SI group. Conclusion: These data suggest the potential of music therapy effects of that listening to music maybe improve the pathological effects of social isolation.