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:: Volume 10, Issue 4 (Autumn 2022) ::
Shefaye Khatam 2022, 10(4): 1-9 Back to browse issues page
The Effect of Four Weeks of Physical Activity on Rac1 Protein Levels and Plasticity of WDR Neurons in the Dorsal Horn of The Spinal Cord in Mice with Experimental Autoimmune Encephalomyelitis
Amir Hossein Saffar Kohneh Quchan, Mohammad Reza Kordi *, Fatemeh Shabkhiz
Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran , mrkordi@ut.ac.ir
Abstract:   (289 Views)
Introduction: Changes in the morphology of dendrites and the function of nerve circuits in pain-sensing neurons in the dorsal horn of the spinal cord ultimately lead to neuropathic pain. Increased expression of Rac1 is associated with increased size and density of dendritic spines and increased pain threshold in multiple sclerosis (MS). This study aimed to investigate the effect of four weeks of physical activity in an enriched environment on Rac1 and structural changes of WDR neurons in the dorsal horn of the spinal cord, and pain sensitivity in the chronic course of experimental autoimmune encephalomyelitis (EAE), an animal model used to study the immunopathogenesis of MS. Materials and Methods: Thirty female C57BL6 mice were randomly divided into three groups. The first group was induced with MOG35-55 peptide (EAE group), the second group received a saline injection (healthy control group), and the third group, after induction with MOG35-55, was placed in the cage of the enriched environment (group living in enriched environment + EAE). On day 30 post-induction (chronic period of the disease), the formalin test assessed pain sensitivity. Then the mice were anesthetized with ketamine and xylazine, and the spinal cord tissue was removed. Rac1 protein levels were measured by immunohistochemistry. The length and density of dendritic spines were assessed using the Golgi-Cox staining. Results: Our findings showed that living in an enriched environment significantly reduced RAC1 levels, the length and density of dendritic spines of WDR neurons in the dorsal horn of the spinal cord, and pain sensitivity compared to the EAE group. Conclusion: Changing the environment and lifestyle to a happy and enriched environment and active lifestyle could reduce pain sensitivity in MS by preventing structural changes in pain-sensing neurons in the dorsal horn of the spinal cord.
Keywords: Neuralgia, Multiple Sclerosis, Neuronal Plasticity, Exercise
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Type of Study: Research --- Open Access, CC-BY-NC | Subject: Neurorehabilation
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Saffar Kohneh Quchan A H, Kordi M R, Shabkhiz F. The Effect of Four Weeks of Physical Activity on Rac1 Protein Levels and Plasticity of WDR Neurons in the Dorsal Horn of The Spinal Cord in Mice with Experimental Autoimmune Encephalomyelitis. Shefaye Khatam 2022; 10 (4) :1-9
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Volume 10, Issue 4 (Autumn 2022) Back to browse issues page
مجله علوم اعصاب شفای خاتم The Neuroscience Journal of Shefaye Khatam
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