Introduction: Parkinson’s disease (PD) is the second most neurodegenerative disorder which is characterized by a progressive loss of dopaminergic neurons in the substantia nigra pars compacta. Clinical symptoms do not appear until approximately 70% of dopaminergic neurons and 80% of the striatal dopaminergic terminals have been lost. Thus, detecting nonclinical factors such as detecting biomarker for PD is necessary. In this study, we evaluate the serum level of Cystatin C as a possible biomarker of PD in 6-hydroxydopamine (6-OHDA)-induced Parkinsonism in rat. Materials and Methods:  Rats were divided into two groups: Parkinson and Control. 6-OHDA was administered by stereotaxic surgery into forebrain bundle. Severity of the Parkinsonism was evaluated by Apomorphine (APO)-induced rotational test at the third and sixth week’s post-surgery. Also, serum level of Cystatin C was measured before surgery and at the third and sixth weeks post-surgery. Results: Although rats of control group didn’t show a significant response to APO, rats of parkinson group showed significant rotations. The rotations at the sixth week’s post-surgery were significantly more than the rotations at the third week’s post-surgery. However, there was no significant difference between serum level of Cystatin C in rats of control and parkinson group. Also, there was no difference between serum level of Cystatin C in rats of parkinson group before and after the surgery. There was no difference between serum level of Cystatin C and severity of symptoms in rats of parkinson group. Conclusion: Our data show that in 6-OHDA animal model of PD, serum level of Cystatin C cannot predict onset or progress of PD and therefore this compound cannot consider as a biomarker for PD.