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:: Volume 12, Issue 2 (Spring 2024) ::
Shefaye Khatam 2024, 12(2): 1-9 Back to browse issues page
The Neuroprotective Effects of Nano-Scaffolds Enriched with Piracetam and Octreotide Drugs on Neural Cells Following Oxidative Stress
Tahereh  Karimi shayan , Asadollah Asadi * , Arash Abdolmaleki , Hossein Hassanpour
Department of Biology, Faculty of Science, University of Mohaghegh Ardabili, Ardabil, Iran , asad.asady@gmail.com
Abstract:   (811 Views)
Introduction: A key step in the success of tissue regeneration is the selection of suitable biomaterials for the preparation of extracellular matrix-mimicking scaffolds. Polycaprolactone (PCL) is used as a scaffold in regenerative therapy and drug delivery applications. The purpose of this study is to develop a PCL nano-scaffold enriched with piracetam and octreotide and to investigate its neuroprotective effects on neural progenitor cells. Materials and Methods: First, drug-enriched nano scaffolds were prepared and their properties were evaluated with different tests. After preparing the electrospun nano-scaffold enriched with piracetam and octreotide drugs, PC12 cells with a density of 1 x 104 cells were planted in the wells of the 96-well plate. After 2 hours, H2O2 was added to the wells to achieve a final concentration of 57 mM. Cell viability was then determined 24 hours later using the MTT method. Results: The morphology of the scaffold and its chemical structure showed appropriate porosity. The biocompatibility of the scaffold, which was checked 24 hours after the cultivation of PC12 cells, showed an increase in the viability of the cells as well as the proper connection of the cells on the scaffold. Conclusion: Our results demonstrated the biocompatibility and non-toxicity of the PCL scaffold, along with increased cell survival on PCL/piracetam and PCL/octreotide scaffolds.
Keywords: Cell Culture Techniques, Extracellular Matrix, Tissue Engineering
Full-Text [PDF 1073 kb]   (177 Downloads)    
Type of Study: Research --- Open Access, CC-BY-NC | Subject: Neurophysiology
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Karimi shayan T, Asadi A, Abdolmaleki A, Hassanpour H. The Neuroprotective Effects of Nano-Scaffolds Enriched with Piracetam and Octreotide Drugs on Neural Cells Following Oxidative Stress. Shefaye Khatam 2024; 12 (2) :1-9
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Volume 12, Issue 2 (Spring 2024) Back to browse issues page
مجله علوم اعصاب شفای خاتم The Neuroscience Journal of Shefaye Khatam
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