[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit ::
Main Menu
Home::
Journal Information::
Articles Archive::
Guide for Authors::
For Reviewers::
Ethical Statements::
Registration::
Site Facilities::
Contact us::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
Copyright Policies

 

AWT IMAGE

 

..
Open Access Policy

This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.

..
:: Volume 2 - The Autumn Supplement of Shefaye Khatam 3 - ::
Shefaye Khatam 2014, 2 - The Autumn Supplement of Shefaye Khatam 3 -: 65-65 Back to browse issues page
P15: Analysis of Receptor’s Distribution in Entorhinal Cortex after Induction of Spreading Depression in Juvenile Rats
Amir Ghaemi * , Ahmad Ali Lotfinia , Leila Alizadeh
a. Department of Microbiology, Golestan University of Medical Sciences, Gorgan, Iran. b. Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran. , ghaem_amir@yahoo.com
Abstract:   (5698 Views)
Spreading depression (SD), discovered by Leao in 1944, is a pathophysiological wave which propagates slowly in the brain (3 mm/min) and cause dramatic ionic and hemodynamic changes. SD appears to act through several mechanisms and receptors which have not completely understood. Here, we studied the effect of inhibitory system in animal model of SD using immunohistochemistry technique. After implanting recording electrodes and cannula over the brain, repetitive SD was induced by KCl injection (2 M) in juvenile rats for four consecutive weeks. Then all rats were decapitated and the brains removed. Mean number of dark neurons in entorhinal cortex were determined using Toluidine blue staining. To identify the prevalence and distribution of γ-aminobutyric acid A (GABA-A) subunit receptors and glutamic acid decarboxylase (GAD), immunohistochemistry technique was performed. The mean number of SD induced by KCl injection was statistically increased during four weeks of experiments (P = 0.036). The mean number of dark neurons in entorhinal cortex was significantly increased in SD group compared to sham rats (P ≤ 0.001). Also, expression of GAD 65 receptor in the Entorhinal cortex significantly increased in SD group compare to control group (P < 0.05). GABA-Aα and GABA-Aβ receptors didn’t show significant change in that region. These data suggest that SD is able to damage neural cells and also it could lead to enhancement of GAD, the enzyme which is responsible for synthesizing an important inhibitory neurotransmitter, GABA receptor, in the central nervous system.
Keywords: Cortical Spreading Depression, Entorhinal Cortex, Gamma-Aminobutyric Acid A
Full-Text [PDF 195 kb]   (1653 Downloads)    
Type of Study: Research --- Open Access, CC-BY-NC | Subject: Basic research in Neuroscience


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ghaemi A, Lotfinia A A, Alizadeh L. P15: Analysis of Receptor’s Distribution in Entorhinal Cortex after Induction of Spreading Depression in Juvenile Rats. Shefaye Khatam 2014; 2 (S3) :65-65
URL: http://shefayekhatam.ir/article-1-524-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 2 - The Autumn Supplement of Shefaye Khatam 3 - Back to browse issues page
مجله علوم اعصاب شفای خاتم The Neuroscience Journal of Shefaye Khatam
Persian site map - English site map - Created in 0.05 seconds with 45 queries by YEKTAWEB 4642