Introduction: Spreading depression (SD) is a bio-electrical wave in the central nervous system which propagates through the gray matter and cause several effects, such as neural depolarization and ionic disturbances. This phenomenon plays a role in many neurological disorders, like epilepsy, migraine with aura, brain injury and cerebrovascular disease. The SD excitatory effect of glutamate receptors on the brain seems to be correlated with neural injuries. The aim of our study was to evaluate the role of glutamate AMPA subreceptors on spatial memory impairment induced by SD. Materials and Methods: DNQX, an AMPA antagonist, was administrated (1 mg/kg) intraperitoneally after SD induction (induced by application of 2 M KCl) in juvenile wistar rats (60-80 gr). Retrieval of spatial memory was evaluated by T-maze memory test. Results: Repetitive weekly induction of SD caused memory impairment after four weeks. Blockade of AMPA receptors with DNQX did not affect the memory impairment caused by SD. Conclusion: Our data indicate glutamate AMPA subreceptors are not involved in SD-induced memory deficits in juvenile rats.