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Effects of Iron on the Neuronal Degeneration of the L4 Dorsal Root Ganglion in an Animal Model of Sciatic Nerve Transection
Somaye Mohammadi Abisofla , Morteza Behnam Rassouli * , Maryam Mousavi nezhad Moghaddam , Naser Mahdavi Shahri
Peripheral Nerve Regeneration Research Lab, Departmentof Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran , behnam@ferdowsi.um.ac.ir
Abstract:   (83 Views)
Introduction: Iron is an essential and potentially toxic metal in the mammalian body. In the present study,  the effects of Ferrous Sulphate as an exogenous iron on the neuronal degeneration induced by unilateral sciatic nerve transection (SNT) in L4 dorsal root ganglion (L4-DRG) have been investigated in rats. Materials and Methods: Twenty-four male Wistar rat were divided into 4 groups (n=6); control (intact), SNT+ Salin, SNT+ iron (5.6 mg/kg; i.p), and sham. Treatment was started 1 hour after injury and continued for 7 days following the injury. The L4-DRG were dissected out, fixed (formalin 10%) and then processed for paraffin embedding 3 weeks after injury. Serial sections of L4-DRGs were prepared, stained (H&E and Toluidine blue) and examined microscopically. The mean volume of L4-DRG was estimated using Cavalieri principle and neuron count was done by a stereological approach (Disector method). Results: The mean number of neurons in SNT and SNT+ iron groups were significantly decreased compared to the control group. In addition, the mean DRG volume in SNT+iron group was significantly lower than control group. Conclusion: Treatment with iron seems to exacerbate the neuronal death induced by SNT in rats.
Keywords: Iron, Sciatic Nerve, Rats
Full-Text [PDF 874 kb]   (21 Downloads)    
Type of Study: Research --- Open Access, CC-BY-NC | Subject: Basic research in Neuroscience


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مجله علوم اعصاب شفای خاتم The Neuroscience Journal of Shefaye Khatam
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