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:: Volume 8, Issue 2 (Spring 2020) ::
Shefaye Khatam 2020, 8(2): 82-92 Back to browse issues page
Production and Evaluation of Anti-Mouse Polyclonal Antibody Against Enterotoxin B of Staphylococcus Aurous
Zeinab Najmi, Soheil Ghasemi, Rohollah Ghalandari, Fattah Sotoudehnejad Nematalahi *
Department of Biology, School of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran , fattah212@gmail.com
Abstract:   (498 Views)
Introduction: Staphylococcus aureus is an important microorganism that causes the development of various diseases in humans by secretion of factors that are known as supra-antigen of staphylococci. Enterotoxin B of Staphylococcus aureus is a bacterial antigen responsible for food poisoning in humans. To produce the corresponding polyclonal antibody, an antigen is injected into a susceptible animal and the serum of antibody content is extracted. Bacterial superantigens are potent T cell activators that can have acute or chronic effects on the central nervous system. This study aimed to develop a mouse polyclonal antibody against enterotoxin B of Staphylococcus aureus. Materials and Methods: The Bradford method was used to determine protein concentration. For evaluation and identification of the antigen, the samples were transferred onto a nitrocellulose membrane by SDS-PAEG gel and analyzed by western blot analysis. Mice immunization was performed at intervals of zero, two, and four weeks using intraperitoneal injection. Antibody titer was measured in antisera isolated from the animal by the ELISA method. Results: Different concentrations of protein (0-32 μg) with different adsorption were calculated with the formula y = 0.0279x + 0.1222. There was no excess protein in the acrylamide gel. In the western blot analysis, the resulting bands represent complete conformance with the standard sample and are free from any unwanted protein. The results of the ELISA test were significant for the secretion of the second time at p <0.05. In the double diffusion test, there was a bond between the control and the antigen. Conclusion: Prepared toxoid has completely lost its fecundity and therefore could be used to immunize and produce polyclonal antibodies against enterotoxin B of Staphylococci.
Keywords: Staphylococcus, Antibodies, Central Nervous System
Full-Text [PDF 1106 kb]   (110 Downloads)    
Type of Study: Research --- Open Access, CC-BY-NC | Subject: Basic research in Neuroscience
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Najmi Z, Ghasemi S, Ghalandari R, Sotoudehnejad Nematalahi F. Production and Evaluation of Anti-Mouse Polyclonal Antibody Against Enterotoxin B of Staphylococcus Aurous. Shefaye Khatam. 2020; 8 (2) :82-92
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Volume 8, Issue 2 (Spring 2020) Back to browse issues page
مجله علوم اعصاب شفای خاتم The Neuroscience Journal of Shefaye Khatam
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