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:: Volume 11, Issue 2 (Spring 2023) ::
Shefaye Khatam 2023, 11(2): 75-80 Back to browse issues page
The Split Well: a New Method for Comparing Nano-Scaffolds in Electron Microscopy
Javad Momeni , Mehdi Hadi Jafari , Hadi Aligholi , Sajad Sahab Negah *
a. Neuroscience Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. b. Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran , Sahabnegahs@mums.ac.ir
Abstract:   (882 Views)
Introduction: Comparing the nanostructure of multiple scaffolds for tissue engineering using electron microscopy poses significant challenges in terms of time and cost. To overcome this, we have developed a novel method that enables the assessment of two different scaffolds on a single slide in scanning electron microscopy (SEM). Materials and Methods: The Pura Matrix scaffold was used to study this method. Firstly, each scaffold was positioned at the bottom of a well, ensuring that every sample was placed on one specific side of the well. Then, each sample was washed with PBS and fixed with glutaraldehyde. After the scaffolds were fixed, they were washed three times with PBS and finally dried overnight at room temperature. After 24 hours, the samples were coated in gold and then observed using SEM. Results: In this study, we utilized this method successfully to investigate the structures of different self-assembling peptides. Our split-well approach not only saves time for researchers and reduces confounding variables but also minimizes the cost of services by allowing simultaneous evaluation of two scaffolds. Conclusion: Our findings demonstrate that researchers can achieve precise comparisons using similar magnifications. Overall, the split-well approach proves to be a cost-effective and accurate method for comparing different scaffolds in tissue engineering.
Keywords: Cell Culture Techniques, Tissue Engineering, Methods
Full-Text [PDF 1389 kb]   (684 Downloads)    
Type of Study: Research --- Open Access, CC-BY-NC | Subject: Basic research in Neuroscience
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Momeni J, Hadi Jafari M, Aligholi H, Sahab Negah S. The Split Well: a New Method for Comparing Nano-Scaffolds in Electron Microscopy. Shefaye Khatam 2023; 11 (2) :75-80
URL: http://shefayekhatam.ir/article-1-2402-en.html
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Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 11, Issue 2 (Spring 2023) Back to browse issues page
مجله علوم اعصاب شفای خاتم The Neuroscience Journal of Shefaye Khatam
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